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• W2769084532 abstract "We report the observation of chromatin dynamics in living budding yeast (Saccharomyces cerevisiae) cells, in three-dimensions (3D). Using dual color localization microscopy and employing a Tetrapod point spread function, we analyze the spatio-temporal dynamics of two fluorescently labeled DNA loci surrounding the GAL locus. From the measured trajectories, we obtain different dynamical characteristics in terms of inter-loci distance and temporal variance; when the GAL locus is activated, the 3D inter-loci distance and temporal variance increase compared to the inactive state. These changes are visible in spite of the large thermally- and biologically-driven heterogeneity in the relative motion of the two loci. Our observations are consistent with current euchromatin vs. heterochromatin models." @default.
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• W2769084532 date "2017-11-21" @default.
• W2769084532 modified "2023-10-12" @default.
• W2769084532 title "Observation of live chromatin dynamics in cells via 3D localization microscopy using Tetrapod point spread functions" @default.
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• W2769084532 doi "https://doi.org/10.1364/boe.8.005735" @default.
• W2769084532 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5745116" @default.
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