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• W2769209214 abstract "Normalization is the first critical step in microbiome sequencing data analysis used to account for variable library sizes. Current RNA-Seq based normalization methods that have been adapted for microbiome data fail to consider the unique characteristics of microbiome data, which contain a vast number of zeros due to the physical absence or under-sampling of the microbes. Normalization methods that specifically address the zero-inflation remain largely undeveloped. Here we propose geometric mean of pairwise ratios—a simple but effective normalization method—for zero-inflated sequencing data such as microbiome data. Simulation studies and real datasets analyses demonstrate that the proposed method is more robust than competing methods, leading to more powerful detection of differentially abundant taxa and higher reproducibility of the relative abundances of taxa." @default.
• W2769209214 created "2017-12-04" @default.
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• W2769209214 date "2018-04-02" @default.
• W2769209214 modified "2023-10-11" @default.
• W2769209214 title "GMPR: A robust normalization method for zero-inflated count data with application to microbiome sequencing data" @default.
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• W2769209214 doi "https://doi.org/10.7717/peerj.4600" @default.
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• W2769209214 hasPublicationYear "2018" @default.
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