FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2769742893> ?p ?o ?g. }

• W2769742893 abstract "Snails are essential for the transmission and maintenance of schistosomiasis in endemic areas, as they serve as intermediate hosts for schistosome parasites. A clear understanding of the snail species present, their local distribution and infection status is therefore a prerequisite for effective control of schistosomiasis. The purpose of this study was to establish the infection status and distribution of Schistosoma mansoni in snails in the Gombe area along the shores of Lake Tanganyika in western Tanzania, using both detection of cercarial shedding and molecular approaches. Snails were collected from streams located close to human settlements in Gombe National Park, as well as from nearby villages (Kiziba, Mtanga, Mwamgongo and Bugamba) and the largest town in the region (Kigoma). Snails were individually exposed to light to induce shedding of schistosome larvae, which were examined using a compound light microscope. Additionally, the internal transcribed spacer (ITS) region of the ribosomal RNA gene cluster was simultaneously amplified in both snails and their trematodes using a single polymerase chain reaction (PCR) and sequenced to confirm species identification. Snails morphologically identified as Biomphalaria pfeifferi were present in all streams except at Mtanga but their distribution was patchy in both time and space. Sequencing of PCR products indicated that not all snails were B. pfeifferi. None of the snails from Gombe or Bugamba shed schistosome larvae, while larvae were shed at all other sites. Overall, an infection prevalence of only 12% was observed in snails based on cercarial shedding. While 47% of the snails were PCR-positive for the 500 bp ITS fragment, which was predicted to indicate infection with S. mansoni, sequence data demonstrated that these bands are not species-specific and can be amplified from other trematode infections. In addition, a 1000 bp band was amplified in 14% of samples, which was identified as a trematode in the family Derogenidae. The results support the previous assumption that B. pfeifferi snails may be involved in transmitting schistosomiasis in the area but suggest that the community structure of both snails and trematodes may be more complicated than previously thought. This emphasises the importance of confirming species identifications using sequencing, rather than relying only on PCR-based diagnostics or cercarial shedding." @default.
• W2769742893 created "2017-12-04" @default.
• W2769742893 creator A5003954577 @default.
• W2769742893 creator A5011391636 @default.
• W2769742893 creator A5054225313 @default.
• W2769742893 creator A5056292776 @default.
• W2769742893 creator A5063246170 @default.
• W2769742893 creator A5078928505 @default.
• W2769742893 date "2017-11-23" @default.
• W2769742893 modified "2023-09-27" @default.
• W2769742893 title "Assessing S. mansoni prevalence in Biomphalaria snails in the Gombe ecosystem of western Tanzania: the importance of DNA sequence data for clarifying species identification" @default.
• W2769742893 cites W141076783 @default.
• W2769742893 cites W1922015448 @default.
• W2769742893 cites W1965236503 @default.
• W2769742893 cites W1972690993 @default.
• W2769742893 cites W1983276011 @default.
• W2769742893 cites W1983948015 @default.
• W2769742893 cites W1994417076 @default.
• W2769742893 cites W1995531577 @default.
• W2769742893 cites W1998902065 @default.
• W2769742893 cites W2000246933 @default.
• W2769742893 cites W2000442686 @default.
• W2769742893 cites W2005660529 @default.
• W2769742893 cites W2007073665 @default.
• W2769742893 cites W2010332240 @default.
• W2769742893 cites W2019485730 @default.
• W2769742893 cites W2023498745 @default.
• W2769742893 cites W2029378619 @default.
• W2769742893 cites W2031031462 @default.
• W2769742893 cites W2041921633 @default.
• W2769742893 cites W2045515993 @default.
• W2769742893 cites W2069064865 @default.
• W2769742893 cites W2078970179 @default.
• W2769742893 cites W2091660523 @default.
• W2769742893 cites W2094078787 @default.
• W2769742893 cites W2096377982 @default.
• W2769742893 cites W2104084526 @default.
• W2769742893 cites W2109328916 @default.
• W2769742893 cites W2109723594 @default.
• W2769742893 cites W2133588732 @default.
• W2769742893 cites W2138632473 @default.
• W2769742893 cites W2140719375 @default.
• W2769742893 cites W2142943181 @default.
• W2769742893 cites W2144278880 @default.
• W2769742893 cites W2147270435 @default.
• W2769742893 cites W2158545629 @default.
• W2769742893 cites W2162821280 @default.
• W2769742893 cites W2167983111 @default.
• W2769742893 cites W2169149548 @default.
• W2769742893 cites W2171894744 @default.
• W2769742893 cites W2175674055 @default.
• W2769742893 cites W2205114462 @default.
• W2769742893 cites W2332453666 @default.
• W2769742893 cites W2469953689 @default.
• W2769742893 cites W2470745861 @default.
• W2769742893 cites W2515887488 @default.
• W2769742893 cites W4247698682 @default.
• W2769742893 cites W4301665628 @default.
• W2769742893 doi "https://doi.org/10.1186/s13071-017-2525-6" @default.
• W2769742893 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5701381" @default.
• W2769742893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29169384" @default.
• W2769742893 hasPublicationYear "2017" @default.
• W2769742893 type Work @default.
• W2769742893 sameAs 2769742893 @default.
• W2769742893 citedByCount "16" @default.
• W2769742893 countsByYear W27697428932020 @default.
• W2769742893 countsByYear W27697428932021 @default.
• W2769742893 countsByYear W27697428932022 @default.
• W2769742893 countsByYear W27697428932023 @default.
• W2769742893 crossrefType "journal-article" @default.
• W2769742893 hasAuthorship W2769742893A5003954577 @default.
• W2769742893 hasAuthorship W2769742893A5011391636 @default.
• W2769742893 hasAuthorship W2769742893A5054225313 @default.
• W2769742893 hasAuthorship W2769742893A5056292776 @default.
• W2769742893 hasAuthorship W2769742893A5063246170 @default.
• W2769742893 hasAuthorship W2769742893A5078928505 @default.
• W2769742893 hasBestOaLocation W27697428931 @default.
• W2769742893 hasConcept C165901193 @default.
• W2769742893 hasConcept C18903297 @default.
• W2769742893 hasConcept C2775934075 @default.
• W2769742893 hasConcept C2776225656 @default.
• W2769742893 hasConcept C2776863245 @default.
• W2769742893 hasConcept C2777445151 @default.
• W2769742893 hasConcept C2778491710 @default.
• W2769742893 hasConcept C2778933494 @default.
• W2769742893 hasConcept C2779965526 @default.
• W2769742893 hasConcept C42972112 @default.
• W2769742893 hasConcept C71924100 @default.
• W2769742893 hasConcept C86803240 @default.
• W2769742893 hasConcept C90856448 @default.
• W2769742893 hasConceptScore W2769742893C165901193 @default.
• W2769742893 hasConceptScore W2769742893C18903297 @default.
• W2769742893 hasConceptScore W2769742893C2775934075 @default.
• W2769742893 hasConceptScore W2769742893C2776225656 @default.
• W2769742893 hasConceptScore W2769742893C2776863245 @default.
• W2769742893 hasConceptScore W2769742893C2777445151 @default.
• W2769742893 hasConceptScore W2769742893C2778491710 @default.
• W2769742893 hasConceptScore W2769742893C2778933494 @default.
• W2769742893 hasConceptScore W2769742893C2779965526 @default.
• W2769742893 hasConceptScore W2769742893C42972112 @default.

• next