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- W2769871212 abstract "Neurofilaments (NFs) are thought to provide stability to the axon. We examined NF dynamics within axonal neurites of NB2a/d1 neuroblastoma by transient transfection with green fluorescent protein-tagged NF-H (GFP-H) under the control of a tetracycline-inducible promoter. Immunofluorescent and biochemical analyses demonstrated that GFP-H expressed early during neurite outgrowth associated with a population of centrally-situated, highly-phosphorylated crosslinked NFs along the length of axonal neurites (“bundled NFs”). By contrast, GFP-H expressed after considerable neurite outgrowth displayed markedly reduced association with bundled NFs and was instead more evenly distributed throughout the axon. This differential localization was maintained for up to 2 weeks in culture. Once considerable neurite outgrowth had progressed, GFP that had previously associated with the NF bundle during early expression was irreversibly depleted by photobleaching. Cessation of expression allowed monitoring of NF turnover. GFP-H associated bundled NFs underwent slower decay than GFP-H associated with surrounding, less-phosphorylated NFs. Notably, GFP associated with bundled NFs underwent similar decay rates within the core and edges of this bundle. These results are consistent with previous demonstration of a resident NF population within axonal neurites, but suggest that this population is more dynamic than previously considered." @default.
- W2769871212 created "2017-12-04" @default.
- W2769871212 creator A5012105133 @default.
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- W2769871212 date "2017-01-01" @default.
- W2769871212 modified "2023-09-27" @default.
- W2769871212 title "Assembly and turnover of neurofilaments in growing axonal neurites" @default.
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- W2769871212 doi "https://doi.org/10.1242/bio.028795" @default.
- W2769871212 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5829495" @default.
- W2769871212 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29158321" @default.
- W2769871212 hasPublicationYear "2017" @default.
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