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• W27699049 abstract "BioTechniquesVol. 28, No. 3 BenchmarksOpen AccessHigh Copy Number Plasmids Compatible with Commonly Used Cloning VectorsGregory J. Phillips, Sei-Kyoung Park & Damon HuberGregory J. Phillips*Address correspondence to Dr. Gregory J. Phillips, Department of Microbiology, 207 Science I Building, Iowa State University, Ames, IA 50011, USA. Internet: E-mail Address: gregory@iastate.eduIowa State University, Ames, IA, USASearch for more papers by this author, Sei-Kyoung ParkIowa State University, Ames, IA, USASearch for more papers by this author & Damon HuberIowa State University, Ames, IA, USASearch for more papers by this authorPublished Online:29 Aug 2018https://doi.org/10.2144/00283bm02AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInReddit FiguresReferencesRelatedDetailsCited ByReducing metabolic burden in the PACEmid evolver system by remastering high‐copy phagemid vectors20 May 2022 | Engineering Biology, Vol. 6, No. 2-3Genetic Encoding of N6 -(((Trimethylsilyl)methoxy)carbonyl)- l -lysine for NMR Studies of Protein–Protein and Protein–Ligand Interactions5 January 2021 | Journal of the American Chemical Society, Vol. 143, No. 2Tuning of the enzyme ratio in a neutral redox convergent cascade: A key approach for an efficient one‐pot/two‐step biocatalytic whole‐cell system3 September 2019 | Biotechnology and Bioengineering, Vol. 116, No. 11Efficient Phage Display with Multiple Distinct Non‐Canonical Amino Acids Using Orthogonal Ribosome‐Mediated Genetic Code Expansion3 July 2019 | Angewandte Chemie, Vol. 131, No. 32Efficient Phage Display with Multiple Distinct Non‐Canonical Amino Acids Using Orthogonal Ribosome‐Mediated Genetic Code ExpansionAngewandte Chemie International Edition, Vol. 58, No. 32Escherichia coli vectors having stringently repressible replication origins allow a streamlining of Crispr/Cas9 gene editingPlasmid, Vol. 103New Insights into Amino-Terminal Translocation as Revealed by the Use of YidC and Sec Depletion StrainsJournal of Molecular Biology, Vol. 431, No. 5pELMO, an optimised in-house cloning vector24 January 2017 | AMB Express, Vol. 7, No. 1Directed evolution of leucine dehydrogenase for improved efficiency of l-tert-leucine synthesis22 February 2016 | Applied Microbiology and Biotechnology, Vol. 100, No. 13Analysis of amino acid substitutions in AraC variants that respond to triacetic acid lactone20 January 2016 | Protein Science, Vol. 25, No. 4The CreC Regulator of Escherichia coli, a New Target for Metabolic ManipulationsApplied and Environmental Microbiology, Vol. 82, No. 1Functional enrichment by direct plasmid recovery after fluorescence activated cell sortingBalakrishnan Ramesh, Christopher S Frei, Patrick C Cirino & Navin Varadarajan3 April 2018 | BioTechniques, Vol. 59, No. 3A series of medium and high copy number arabinose-inducible Escherichia coli expression vectors compatible with pBR322 and pACYC184Plasmid, Vol. 81Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesisMetabolic Engineering, Vol. 30Improvement of NADPH bioavailability in Escherichia coli by replacing NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase GapA with NADP+-dependent GapB from Bacillus subtilis and addition of NAD kinase1 December 2013 | Journal of Industrial Microbiology and Biotechnology, Vol. 40, No. 12Metabolic engineering and transhydrogenase effects on NADPH availability in escherichia coli22 June 2013 | Biotechnology Progress, Vol. 29, No. 5Improvement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strains5 April 2013 | Applied Microbiology and Biotechnology, Vol. 97, No. 15Protein-Protein Interactions in Assembly of Lipoic Acid on the 2-Oxoacid Dehydrogenases of Aerobic MetabolismJournal of Biological Chemistry, Vol. 286, No. 10Mercury(II)-resistance transposons Tn502 and Tn512, from Pseudomonas clinical strains, are structurally different members of the Tn5053 familyPlasmid, Vol. 65, No. 1Tn 502 and Tn 512 Are res Site Hunters That Provide Evidence of Resolvase-Independent Transposition to Random SitesJournal of Bacteriology, Vol. 192, No. 7Metabolic engineering of the anaerobic central metabolic pathway in Escherichia coli for the simultaneous anaerobic production of isoamyl acetate and succinic acid22 September 2009 | Biotechnology Progress, Vol. 25, No. 5Chapter 17 Bacterial Fatty Acid Synthesis and its Relationships with Polyketide Synthetic PathwaysReplacing Escherichia coli NAD-dependent glyceraldehyde 3-phosphate dehydrogenase (GAPDH) with a NADP-dependent enzyme from Clostridium acetobutylicum facilitates NADPH dependent pathwaysMetabolic Engineering, Vol. 10, No. 6Strict regulation of gene expression from a high-copy plasmid utilizing a dual vector systemProtein Expression and Purification, Vol. 60, No. 1New pSC101-derivative cloning vectors with elevated copy numbersPlasmid, Vol. 59, No. 3AraC Regulatory Protein Mutants with Altered Effector Specificity21 March 2008 | Journal of the American Chemical Society, Vol. 130, No. 15Engineering poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer composition inE. coli1 January 2008 | Biotechnology and Bioengineering, Vol. 99, No. 4Selection and characterization of Yersinia pestis YopN mutants that constitutively block Yop secretion30 June 2005 | Molecular Microbiology, Vol. 57, No. 4Effect of different levels of NADH availability on metabolic fluxes of Escherichia coli chemostat cultures in defined mediumJournal of Biotechnology, Vol. 117, No. 4Novel pathway engineering design of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivityMetabolic Engineering, Vol. 7, No. 3HIV-1 Vif Can Directly Inhibit Apolipoprotein B mRNA-editing Enzyme Catalytic Polypeptide-like 3G-mediated Cytidine Deamination by Using a Single Amino Acid Interaction and Without Protein DegradationJournal of Biological Chemistry, Vol. 280, No. 10General co-expression vectors for the overexpression of heterodimeric protein complexes in Escherichia coliProtein Expression and Purification, Vol. 38, No. 1Applicability of CoA/acetyl-CoA manipulation system to enhance isoamyl acetate production in Escherichia coliMetabolic Engineering, Vol. 6, No. 4Effect of different levels of NADH availability on metabolite distribution in Escherichia coli fermentation in minimal and complex media7 April 2004 | Applied Microbiology and Biotechnology, Vol. 65, No. 4High-level production and optimization of monodispersity of 11β-hydroxysteroid dehydrogenase type 1Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1700, No. 2Industrial scale production of plasmid DNA for vaccine and gene therapy: plasmid design, production, and purificationEnzyme and Microbial Technology, Vol. 33, No. 7Cosmid-Based System for Transient Expression and Absolute Off-to-On Transcriptional Control of Escherichia coli GenesJournal of Bacteriology, Vol. 185, No. 22Gene replacement without selection: regulated suppression of amber mutations in Escherichia coliGene, Vol. 311Expression of a Cloned Cyclopropane Fatty Acid Synthase Gene Reduces Solvent Formation in Clostridium acetobutylicum ATCC 824Applied and Environmental Microbiology, Vol. 69, No. 5Functional Analysis of the Signal Recognition Particle in Escherichia coli by Characterization of a Temperature-Sensitive ffh MutantJournal of Bacteriology, Vol. 184, No. 10 Vol. 28, No. 3 Follow us on social media for the latest updates Metrics Downloaded 3,881 times History Published online 29 August 2018 Published in print March 2000 Information© 2018 Author(s)PDF download" @default.
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