FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2769916510> ?p ?o ?g. }

• W2769916510 endingPage "1943" @default.
• W2769916510 startingPage "1933" @default.
• W2769916510 abstract "The human ether-a-go-go related gene (hERG)–encoded channel hERG undergoes N-linked glycosylation at position 598, which is located in the unusually long S5-pore linker of the channel. In other work we have demonstrated that hERG is uniquely susceptible to proteolytic cleavage at the S5-pore linker by proteinase K (PK) and calpain (CAPN). The scorpion toxin BeKm-1, which binds to the S5-pore linker of hERG, protects hERG from such cleavage. In the present study, our data revealed that, compared with normal glycosylated hERG channels, nonglycosylated hERG channels were significantly more susceptible to cleavage by extracellular PK. Furthermore, the protective effect of BeKm-1 on hERG from PK-cleavage was lost when glycosylation of hERG was inhibited. The inactivation-deficient mutant hERG channels S620T and S631A were resistant to PK cleavage, and inhibition of glycosylation rendered both mutants susceptible to PK cleavage. Compared with normal glycosylated channels, nonglycosylated hERG channels were also more susceptible to cleavage mediated by CAPN, which was present in the medium of human embryonic kidney cells under normal culture conditions. Inhibition of CAPN resulted in an increase of nonglycosylated hERG current. In summary, our results revealed that N-linked glycosylation protects hERG against protease-mediated degradation and thus contributes to hERG channel stability on the plasma membrane.— Lamothe, S. M., Hulbert, M., Guo, J., Li, W., Yang, T., Zhang, S. Glycosylation stabilizes hERG channels on the plasma membrane by decreasing proteolytic susceptibility. FASEB J. 32, 1933–1943 (2018). www.fasebj.org" @default.
• W2769916510 created "2017-12-04" @default.
• W2769916510 creator A5011343099 @default.
• W2769916510 creator A5023735912 @default.
• W2769916510 creator A5061837237 @default.
• W2769916510 creator A5069337184 @default.
• W2769916510 creator A5069550460 @default.
• W2769916510 creator A5083617730 @default.
• W2769916510 date "2018-01-05" @default.
• W2769916510 modified "2023-10-03" @default.
• W2769916510 title "Glycosylation stabilizes hERG channels on the plasma membrane by decreasing proteolytic susceptibility" @default.
• W2769916510 cites W1523753692 @default.
• W2769916510 cites W1570113951 @default.
• W2769916510 cites W1736358599 @default.
• W2769916510 cites W1933558125 @default.
• W2769916510 cites W1968098810 @default.
• W2769916510 cites W1968374445 @default.
• W2769916510 cites W1969704929 @default.
• W2769916510 cites W1971653265 @default.
• W2769916510 cites W1981877077 @default.
• W2769916510 cites W1985372182 @default.
• W2769916510 cites W1988457468 @default.
• W2769916510 cites W1989313129 @default.
• W2769916510 cites W1997940647 @default.
• W2769916510 cites W1999864002 @default.
• W2769916510 cites W2007236926 @default.
• W2769916510 cites W2009422836 @default.
• W2769916510 cites W2012168396 @default.
• W2769916510 cites W2015540983 @default.
• W2769916510 cites W2021438486 @default.
• W2769916510 cites W2033401038 @default.
• W2769916510 cites W2033861231 @default.
• W2769916510 cites W2038957929 @default.
• W2769916510 cites W2045610340 @default.
• W2769916510 cites W2045626047 @default.
• W2769916510 cites W2046034976 @default.
• W2769916510 cites W2052976216 @default.
• W2769916510 cites W2056576838 @default.
• W2769916510 cites W2060564098 @default.
• W2769916510 cites W2074705578 @default.
• W2769916510 cites W2076243190 @default.
• W2769916510 cites W2078409863 @default.
• W2769916510 cites W2082435269 @default.
• W2769916510 cites W2085048839 @default.
• W2769916510 cites W2097769598 @default.
• W2769916510 cites W2119748918 @default.
• W2769916510 cites W2119930943 @default.
• W2769916510 cites W2121217914 @default.
• W2769916510 cites W2126688997 @default.
• W2769916510 cites W2142360679 @default.
• W2769916510 cites W2148724942 @default.
• W2769916510 cites W2152815385 @default.
• W2769916510 cites W2164311861 @default.
• W2769916510 cites W2267243801 @default.
• W2769916510 cites W2462490002 @default.
• W2769916510 cites W2496445900 @default.
• W2769916510 cites W2745072699 @default.
• W2769916510 cites W324903366 @default.
• W2769916510 doi "https://doi.org/10.1096/fj.201700832r" @default.
• W2769916510 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32172531" @default.
• W2769916510 hasPublicationYear "2018" @default.
• W2769916510 type Work @default.
• W2769916510 sameAs 2769916510 @default.
• W2769916510 citedByCount "11" @default.
• W2769916510 countsByYear W27699165102018 @default.
• W2769916510 countsByYear W27699165102019 @default.
• W2769916510 countsByYear W27699165102020 @default.
• W2769916510 countsByYear W27699165102021 @default.
• W2769916510 countsByYear W27699165102022 @default.
• W2769916510 countsByYear W27699165102023 @default.
• W2769916510 crossrefType "journal-article" @default.
• W2769916510 hasAuthorship W2769916510A5011343099 @default.
• W2769916510 hasAuthorship W2769916510A5023735912 @default.
• W2769916510 hasAuthorship W2769916510A5061837237 @default.
• W2769916510 hasAuthorship W2769916510A5069337184 @default.
• W2769916510 hasAuthorship W2769916510A5069550460 @default.
• W2769916510 hasAuthorship W2769916510A5083617730 @default.
• W2769916510 hasConcept C111919701 @default.
• W2769916510 hasConcept C12554922 @default.
• W2769916510 hasConcept C151730666 @default.
• W2769916510 hasConcept C170493617 @default.
• W2769916510 hasConcept C175156509 @default.
• W2769916510 hasConcept C181199279 @default.
• W2769916510 hasConcept C185592680 @default.
• W2769916510 hasConcept C2777313579 @default.
• W2769916510 hasConcept C2779362680 @default.
• W2769916510 hasConcept C2780557392 @default.
• W2769916510 hasConcept C2781307694 @default.
• W2769916510 hasConcept C41008148 @default.
• W2769916510 hasConcept C43369102 @default.
• W2769916510 hasConcept C50254741 @default.
• W2769916510 hasConcept C55493867 @default.
• W2769916510 hasConcept C83743174 @default.
• W2769916510 hasConcept C86803240 @default.
• W2769916510 hasConceptScore W2769916510C111919701 @default.
• W2769916510 hasConceptScore W2769916510C12554922 @default.
• W2769916510 hasConceptScore W2769916510C151730666 @default.
• W2769916510 hasConceptScore W2769916510C170493617 @default.

• next