Matches in SemOpenAlex for { <https://semopenalex.org/work/W2769929654> ?p ?o ?g. }
- W2769929654 endingPage "1175" @default.
- W2769929654 startingPage "1169" @default.
- W2769929654 abstract "This work describes purification of a protease from the visceral mass of the mussel Mytella charruana as well as evaluation of its ability to hydrolyze milk casein to generate antimicrobial peptides. The enzyme showed pI of 4.1 and a single polypeptide band of 83.1 kDa after SDS-PAGE. Sequence similarities with tropomyosin and myosin from mollusks were detected. The protease showed a trypsin-like activity with optimal temperature of 40 °C and stability in a wide pH range (3.0-9.0). Km was 4.28 ± 0.34 mM of the synthetic substrate N-benzoyl-dl-arginyl-ρ-nitroanilide, whereas Vmax was 0.056 ± 0.001 nmol min-1. The enzyme hydrolyzed casein, and the hydrolysate inhibited the growth of Escherichia coli, Micrococcus luteus, Bacillus subtilis, and Klebsiella pneumoniae at a minimal inhibitory concentration of 5.0 µg mL-1. In conclusion, the visceral mass of M. charruana contains a trypsin-like protease that can generate peptides from casein that have a bacteriostatic effect." @default.
- W2769929654 created "2017-12-04" @default.
- W2769929654 creator A5018827874 @default.
- W2769929654 creator A5022012723 @default.
- W2769929654 creator A5025622046 @default.
- W2769929654 creator A5041174947 @default.
- W2769929654 creator A5046510304 @default.
- W2769929654 creator A5049718029 @default.
- W2769929654 creator A5053237878 @default.
- W2769929654 creator A5056400493 @default.
- W2769929654 creator A5069273645 @default.
- W2769929654 creator A5086637580 @default.
- W2769929654 creator A5087853484 @default.
- W2769929654 date "2018-04-01" @default.
- W2769929654 modified "2023-10-15" @default.
- W2769929654 title "Purification and characterization of a protease from the visceral mass of Mytella charruana and its evaluation to obtain antimicrobial peptides" @default.
- W2769929654 cites W1094360208 @default.
- W2769929654 cites W1555766099 @default.
- W2769929654 cites W1775749144 @default.
- W2769929654 cites W1971579283 @default.
- W2769929654 cites W1973065099 @default.
- W2769929654 cites W1979494452 @default.
- W2769929654 cites W1980333099 @default.
- W2769929654 cites W1981490451 @default.
- W2769929654 cites W1991960803 @default.
- W2769929654 cites W1995475849 @default.
- W2769929654 cites W2006280533 @default.
- W2769929654 cites W2023059850 @default.
- W2769929654 cites W2033801612 @default.
- W2769929654 cites W2039864978 @default.
- W2769929654 cites W2045520311 @default.
- W2769929654 cites W2062544445 @default.
- W2769929654 cites W2075503331 @default.
- W2769929654 cites W2079733475 @default.
- W2769929654 cites W2081770308 @default.
- W2769929654 cites W2086162518 @default.
- W2769929654 cites W2087019885 @default.
- W2769929654 cites W2100837269 @default.
- W2769929654 cites W2101004007 @default.
- W2769929654 cites W2105041556 @default.
- W2769929654 cites W2130404486 @default.
- W2769929654 cites W2188211649 @default.
- W2769929654 cites W2283330150 @default.
- W2769929654 cites W2285205597 @default.
- W2769929654 cites W2329654044 @default.
- W2769929654 cites W2340275254 @default.
- W2769929654 cites W2464074067 @default.
- W2769929654 cites W2560608117 @default.
- W2769929654 cites W2566071262 @default.
- W2769929654 cites W2571127178 @default.
- W2769929654 cites W2511921319 @default.
- W2769929654 doi "https://doi.org/10.1016/j.foodchem.2017.11.044" @default.
- W2769929654 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29287337" @default.
- W2769929654 hasPublicationYear "2018" @default.
- W2769929654 type Work @default.
- W2769929654 sameAs 2769929654 @default.
- W2769929654 citedByCount "10" @default.
- W2769929654 countsByYear W27699296542018 @default.
- W2769929654 countsByYear W27699296542020 @default.
- W2769929654 countsByYear W27699296542021 @default.
- W2769929654 countsByYear W27699296542022 @default.
- W2769929654 countsByYear W27699296542023 @default.
- W2769929654 crossrefType "journal-article" @default.
- W2769929654 hasAuthorship W2769929654A5018827874 @default.
- W2769929654 hasAuthorship W2769929654A5022012723 @default.
- W2769929654 hasAuthorship W2769929654A5025622046 @default.
- W2769929654 hasAuthorship W2769929654A5041174947 @default.
- W2769929654 hasAuthorship W2769929654A5046510304 @default.
- W2769929654 hasAuthorship W2769929654A5049718029 @default.
- W2769929654 hasAuthorship W2769929654A5053237878 @default.
- W2769929654 hasAuthorship W2769929654A5056400493 @default.
- W2769929654 hasAuthorship W2769929654A5069273645 @default.
- W2769929654 hasAuthorship W2769929654A5086637580 @default.
- W2769929654 hasAuthorship W2769929654A5087853484 @default.
- W2769929654 hasBestOaLocation W27699296541 @default.
- W2769929654 hasConcept C104317684 @default.
- W2769929654 hasConcept C178790620 @default.
- W2769929654 hasConcept C181199279 @default.
- W2769929654 hasConcept C185592680 @default.
- W2769929654 hasConcept C2776142715 @default.
- W2769929654 hasConcept C2776683811 @default.
- W2769929654 hasConcept C2776714187 @default.
- W2769929654 hasConcept C2777118263 @default.
- W2769929654 hasConcept C2777272437 @default.
- W2769929654 hasConcept C2779281246 @default.
- W2769929654 hasConcept C2780340462 @default.
- W2769929654 hasConcept C28800991 @default.
- W2769929654 hasConcept C43617362 @default.
- W2769929654 hasConcept C4937899 @default.
- W2769929654 hasConcept C523546767 @default.
- W2769929654 hasConcept C540938839 @default.
- W2769929654 hasConcept C54355233 @default.
- W2769929654 hasConcept C547475151 @default.
- W2769929654 hasConcept C55493867 @default.
- W2769929654 hasConcept C58121356 @default.
- W2769929654 hasConcept C86803240 @default.
- W2769929654 hasConcept C94412978 @default.
- W2769929654 hasConceptScore W2769929654C104317684 @default.