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• W2770116199 abstract "Recent studies point to the existence of poorly characterized small regulatory RNAs generated from mRNAs, rRNAs and tRNAs. To explore the subcellular location of tRNA-derived small RNAs, 0–1 and 7–8 h Drosophila embryos were fractionated on sucrose density gradients. Analysis of 12,553,921 deep-sequencing reads from unfractionated and fractionated Drosophila embryos has revealed that tRFs, which are detected mainly from the 5’ends of tRNAs, co-sediment with the non-polysomal fractions. Interestingly, the expression levels of a subset of tRFs change temporally following the maternal-to-zygotic transition in embryos. We detected non-polysomal association of tRFs in S2 cells as well. Differential tRF expression pattern points to developmental significance at the organismal level. These results suggest that tRFs are associated primarily with the non-polysomal complexes in Drosophila embryos and S2 cells." @default.
• W2770116199 created "2017-12-04" @default.
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• W2770116199 date "2017-11-20" @default.
• W2770116199 modified "2023-10-01" @default.
• W2770116199 title "Differentially Expressed tRNA-Derived Small RNAs Co-Sediment Primarily with Non-Polysomal Fractions in Drosophila" @default.
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• W2770116199 doi "https://doi.org/10.3390/genes8110333" @default.
• W2770116199 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5704246" @default.
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