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• W2770163029 endingPage "231" @default.
• W2770163029 startingPage "220" @default.
• W2770163029 abstract "The exact function of the polybasic juxtamembrane region (5RK) of the plasma membrane neuronal SNARE, syntaxin 1A (Syx), in vesicle exocytosis, although widely studied, is currently not clear. Here, we addressed the role of 5RK in Ca 2+ -triggered release, using our Syx-based intramolecular fluorescence resonance energy transfer (FRET) probe, which previously allowed us to resolve a depolarization-induced Ca 2+ -dependent close-to-open transition (CDO) of Syx that occurs concomitant with evoked release, both in PC12 cells and hippocampal neurons and was abolished upon charge neutralization of 5RK. First, using dynamic FRET analysis in PC12 cells, we show that CDO occurs following assembly of SNARE complexes that include the vesicular SNARE, synaptobrevin 2, and that the participation of 5RK in CDO goes beyond its participation in the final zippering of the complex, because mutations of residues adjacent to 5RK, believed to be crucial for final zippering, do not abolish this transition. In addition, we show that CDO is contingent on membrane phosphatidylinositol 4,5-bisphosphate (PIP2), which is fundamental for maintaining regulated exocytosis, as depletion of membranal PIP2 abolishes CDO. Prompted by these results, which underscore a potentially significant role of 5RK in exocytosis, we next amperometrically analyzed catecholamine release from PC12 cells, revealing that charge neutralization of 5RK promotes spontaneous and inhibits Ca 2+ -triggered release events. Namely, 5RK acts as a fusion clamp, making release dependent on stimulation by Ca 2+ . SIGNIFICANCE STATEMENT Syntaxin 1A (Syx) is a central protein component of the SNARE complex, which underlies neurotransmitter release. Although widely studied in relation to its participation in SNARE complex formation and its interaction with phosphoinositides, the function of Syx's polybasic juxtamembrane region (5RK) remains unclear. Previously, we showed that a conformational transition of Syx, related to calcium-triggered release, reported by a Syx-based FRET probe, is abolished upon charge neutralization of 5RK (5RK/A). Here we show that this conformational transition is dependent on phosphatidylinositol 4,5-bisphosphate (PIP2) and is related to SNARE complex formation. Subsequently, we show that the 5RK/A mutation enhances spontaneous release and inhibits calcium-triggered release in neuroendocrine cells, indicating a previously unrecognized role of 5RK in neurotransmitter release." @default.
• W2770163029 created "2017-12-04" @default.
• W2770163029 creator A5005335159 @default.
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• W2770163029 creator A5086149496 @default.
• W2770163029 date "2017-11-13" @default.
• W2770163029 modified "2023-10-17" @default.
• W2770163029 title "The Dual Function of the Polybasic Juxtamembrane Region of Syntaxin 1A in Clamping Spontaneous Release and Stimulating Ca²⁺-Triggered Release in Neuroendocrine Cells" @default.
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• W2770163029 doi "https://doi.org/10.1523/jneurosci.1541-17.2017" @default.
• W2770163029 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6705805" @default.
• W2770163029 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29133430" @default.
• W2770163029 hasPublicationYear "2017" @default.
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