FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2770166204> ?p ?o ?g. }

• W2770166204 endingPage "704" @default.
• W2770166204 startingPage "694" @default.
• W2770166204 abstract "Abstract Metabolite production through a multistep metabolic pathway can often be increased by efficient substrate channeling created by spatial sequestration of the metabolic reactions. Here, Tya, a structural component in the Ty1 retrotransposon element that forms virus‐like particles (VLPs) in Saccharomyces cerevisiae , was used to spatially organize enzymes involved in a metabolic pathway into a multi‐enzyme protein body in yeast. As a proof of principle, Tya fusion to three key enzymes involved in biosynthesis of the isoprenoids farnesene and farnesol was tested to assess its potential to improve productivity. The Tya ‐ fusion protein resulted in three and fourfold increases in farnesene and farnesol production, respectively, as compared with that observed in a non‐fused control. Specifically, two‐phase partitioning fed‐batch fermentations of S. cerevisiae ATCC200589 overexpressing Tya‐fused enzymes (tHmg1, IspA, and α‐farnesene synthase) yielded 930 ± 40 mg/L of farnesene after 7 days. Additionally, we observed that the Tya‐fusion proteins tended to partition into particulate fractions upon 100,000 g ultracentrifugation, suggesting the formation of large aggregates of protein bodies, with their particulate structure also observed by transmission electron microscopy. The dramatic increase in the biosynthetic productivity of metabolites via use of a Tya‐fusion protein suggested that this approach might be useful for the creation of multi‐enzyme complexes to improve metabolic engineering in yeast." @default.
• W2770166204 created "2017-12-04" @default.
• W2770166204 creator A5013047009 @default.
• W2770166204 creator A5031509308 @default.
• W2770166204 creator A5035558101 @default.
• W2770166204 creator A5043686584 @default.
• W2770166204 creator A5048380235 @default.
• W2770166204 creator A5052719405 @default.
• W2770166204 creator A5061751953 @default.
• W2770166204 creator A5072867039 @default.
• W2770166204 date "2017-11-27" @default.
• W2770166204 modified "2023-09-29" @default.
• W2770166204 title "Ty1-fused protein-body formation for spatial organization of metabolic pathways in<i>Saccharomyces cerevisiae</i>" @default.
• W2770166204 cites W1483456869 @default.
• W2770166204 cites W1542305002 @default.
• W2770166204 cites W1638707894 @default.
• W2770166204 cites W1964179603 @default.
• W2770166204 cites W1968212788 @default.
• W2770166204 cites W1973494721 @default.
• W2770166204 cites W1974736760 @default.
• W2770166204 cites W1994144928 @default.
• W2770166204 cites W1998379543 @default.
• W2770166204 cites W2018668159 @default.
• W2770166204 cites W2018939181 @default.
• W2770166204 cites W2041681786 @default.
• W2770166204 cites W2042638652 @default.
• W2770166204 cites W2046457549 @default.
• W2770166204 cites W2050933887 @default.
• W2770166204 cites W2056036907 @default.
• W2770166204 cites W2058927469 @default.
• W2770166204 cites W2072185285 @default.
• W2770166204 cites W2077837797 @default.
• W2770166204 cites W2079574042 @default.
• W2770166204 cites W2087118474 @default.
• W2770166204 cites W2103747393 @default.
• W2770166204 cites W2141505745 @default.
• W2770166204 cites W2150895408 @default.
• W2770166204 cites W2166255679 @default.
• W2770166204 cites W2174272296 @default.
• W2770166204 cites W2281020257 @default.
• W2770166204 cites W2399713053 @default.
• W2770166204 cites W2511831309 @default.
• W2770166204 doi "https://doi.org/10.1002/bit.26493" @default.
• W2770166204 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29131321" @default.
• W2770166204 hasPublicationYear "2017" @default.
• W2770166204 type Work @default.
• W2770166204 sameAs 2770166204 @default.
• W2770166204 citedByCount "16" @default.
• W2770166204 countsByYear W27701662042019 @default.
• W2770166204 countsByYear W27701662042020 @default.
• W2770166204 countsByYear W27701662042021 @default.
• W2770166204 countsByYear W27701662042022 @default.
• W2770166204 countsByYear W27701662042023 @default.
• W2770166204 crossrefType "journal-article" @default.
• W2770166204 hasAuthorship W2770166204A5013047009 @default.
• W2770166204 hasAuthorship W2770166204A5031509308 @default.
• W2770166204 hasAuthorship W2770166204A5035558101 @default.
• W2770166204 hasAuthorship W2770166204A5043686584 @default.
• W2770166204 hasAuthorship W2770166204A5048380235 @default.
• W2770166204 hasAuthorship W2770166204A5052719405 @default.
• W2770166204 hasAuthorship W2770166204A5061751953 @default.
• W2770166204 hasAuthorship W2770166204A5072867039 @default.
• W2770166204 hasConcept C104317684 @default.
• W2770166204 hasConcept C123894998 @default.
• W2770166204 hasConcept C147816474 @default.
• W2770166204 hasConcept C181199279 @default.
• W2770166204 hasConcept C185592680 @default.
• W2770166204 hasConcept C192989942 @default.
• W2770166204 hasConcept C2777477808 @default.
• W2770166204 hasConcept C2777576037 @default.
• W2770166204 hasConcept C2779222958 @default.
• W2770166204 hasConcept C40767141 @default.
• W2770166204 hasConcept C553450214 @default.
• W2770166204 hasConcept C55493867 @default.
• W2770166204 hasConcept C6350086 @default.
• W2770166204 hasConceptScore W2770166204C104317684 @default.
• W2770166204 hasConceptScore W2770166204C123894998 @default.
• W2770166204 hasConceptScore W2770166204C147816474 @default.
• W2770166204 hasConceptScore W2770166204C181199279 @default.
• W2770166204 hasConceptScore W2770166204C185592680 @default.
• W2770166204 hasConceptScore W2770166204C192989942 @default.
• W2770166204 hasConceptScore W2770166204C2777477808 @default.
• W2770166204 hasConceptScore W2770166204C2777576037 @default.
• W2770166204 hasConceptScore W2770166204C2779222958 @default.
• W2770166204 hasConceptScore W2770166204C40767141 @default.
• W2770166204 hasConceptScore W2770166204C553450214 @default.
• W2770166204 hasConceptScore W2770166204C55493867 @default.
• W2770166204 hasConceptScore W2770166204C6350086 @default.
• W2770166204 hasFunder F4320322033 @default.
• W2770166204 hasFunder F4320322120 @default.
• W2770166204 hasIssue "3" @default.
• W2770166204 hasLocation W27701662041 @default.
• W2770166204 hasLocation W27701662042 @default.
• W2770166204 hasOpenAccess W2770166204 @default.
• W2770166204 hasPrimaryLocation W27701662041 @default.
• W2770166204 hasRelatedWork W148920908 @default.
• W2770166204 hasRelatedWork W1584603485 @default.
• W2770166204 hasRelatedWork W1971080541 @default.

• next