Matches in SemOpenAlex for { <https://semopenalex.org/work/W2770296654> ?p ?o ?g. }
- W2770296654 endingPage "1188" @default.
- W2770296654 startingPage "1182" @default.
- W2770296654 abstract "Six mutants bearing single amino acid substitutions in the small subunit of Bacillus licheniformis γ-glutamyltranspeptudase (BlGGT) have been constructed by site-directed mutagenesis. The resultant enzymes were overexpressed in Escherichia coli and purified by affinity chromatography for biochemical and biophysical characterizations. Replacing Gly481 by either Ala or Glu did affect both autocatalytic processing and catalytic activity of the enzyme, but the substitution of this residue to arginine resulted in an unprocessed enzyme with insignificant catalytic activity. The replacement of another conserved glycine residue, Gly482, by either Ala or Glu caused a significant change in the functional integrity of the enzyme. Moreover, the mutation of Gly482 to arginine led to a marked reduction in the autocatalytic processing. Structural analyses revealed that the fluorescence and circular dichroism properties of mutant proteins were basically consistent with those of BlGGT. However, guanidine hydrochloride (GdnHCl)-induced transitions of most mutants were profoundly reduced in comparison with that of wild-type enzyme. Molecular modeling suggests that the conserved Gly481 and Gly482 residues of BlGGT are located at critical positions to create an environment suitable for both autoprocessing and catalytic reactions." @default.
- W2770296654 created "2017-12-04" @default.
- W2770296654 creator A5001851397 @default.
- W2770296654 creator A5009946253 @default.
- W2770296654 creator A5018263412 @default.
- W2770296654 creator A5055243163 @default.
- W2770296654 creator A5068294625 @default.
- W2770296654 date "2018-04-01" @default.
- W2770296654 modified "2023-10-16" @default.
- W2770296654 title "Functional role of the conserved glycine residues, Gly481 and Gly482, of the γ-glutamyltranspeptidase from Bacillus licheniformis" @default.
- W2770296654 cites W1494935948 @default.
- W2770296654 cites W1505803621 @default.
- W2770296654 cites W1509576503 @default.
- W2770296654 cites W1578341309 @default.
- W2770296654 cites W1601402371 @default.
- W2770296654 cites W1633567371 @default.
- W2770296654 cites W1967933318 @default.
- W2770296654 cites W1973088164 @default.
- W2770296654 cites W1980919266 @default.
- W2770296654 cites W1984701286 @default.
- W2770296654 cites W1996401827 @default.
- W2770296654 cites W1999736156 @default.
- W2770296654 cites W2002557725 @default.
- W2770296654 cites W2003078070 @default.
- W2770296654 cites W2006373747 @default.
- W2770296654 cites W2008416160 @default.
- W2770296654 cites W2012227432 @default.
- W2770296654 cites W2020198006 @default.
- W2770296654 cites W2022475786 @default.
- W2770296654 cites W2026603700 @default.
- W2770296654 cites W2029486217 @default.
- W2770296654 cites W2036395398 @default.
- W2770296654 cites W2041519330 @default.
- W2770296654 cites W2042887558 @default.
- W2770296654 cites W2044320282 @default.
- W2770296654 cites W2047189235 @default.
- W2770296654 cites W2053448808 @default.
- W2770296654 cites W2053715143 @default.
- W2770296654 cites W2060810897 @default.
- W2770296654 cites W2063268578 @default.
- W2770296654 cites W2069348062 @default.
- W2770296654 cites W2077603638 @default.
- W2770296654 cites W2078394930 @default.
- W2770296654 cites W2083368345 @default.
- W2770296654 cites W2083963344 @default.
- W2770296654 cites W2088610032 @default.
- W2770296654 cites W2088749908 @default.
- W2770296654 cites W2090792051 @default.
- W2770296654 cites W2094148591 @default.
- W2770296654 cites W2094907051 @default.
- W2770296654 cites W2095210131 @default.
- W2770296654 cites W2127689330 @default.
- W2770296654 cites W2140505352 @default.
- W2770296654 cites W2152301430 @default.
- W2770296654 cites W2153338337 @default.
- W2770296654 cites W2157224977 @default.
- W2770296654 cites W2163029410 @default.
- W2770296654 cites W2401503346 @default.
- W2770296654 cites W74371005 @default.
- W2770296654 doi "https://doi.org/10.1016/j.ijbiomac.2017.11.116" @default.
- W2770296654 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29162462" @default.
- W2770296654 hasPublicationYear "2018" @default.
- W2770296654 type Work @default.
- W2770296654 sameAs 2770296654 @default.
- W2770296654 citedByCount "4" @default.
- W2770296654 countsByYear W27702966542019 @default.
- W2770296654 countsByYear W27702966542021 @default.
- W2770296654 countsByYear W27702966542022 @default.
- W2770296654 crossrefType "journal-article" @default.
- W2770296654 hasAuthorship W2770296654A5001851397 @default.
- W2770296654 hasAuthorship W2770296654A5009946253 @default.
- W2770296654 hasAuthorship W2770296654A5018263412 @default.
- W2770296654 hasAuthorship W2770296654A5055243163 @default.
- W2770296654 hasAuthorship W2770296654A5068294625 @default.
- W2770296654 hasConcept C103408237 @default.
- W2770296654 hasConcept C104317684 @default.
- W2770296654 hasConcept C133571119 @default.
- W2770296654 hasConcept C143065580 @default.
- W2770296654 hasConcept C161790260 @default.
- W2770296654 hasConcept C16318435 @default.
- W2770296654 hasConcept C181199279 @default.
- W2770296654 hasConcept C185592680 @default.
- W2770296654 hasConcept C2776414213 @default.
- W2770296654 hasConcept C2776527165 @default.
- W2770296654 hasConcept C2777272437 @default.
- W2770296654 hasConcept C2777468819 @default.
- W2770296654 hasConcept C2779463914 @default.
- W2770296654 hasConcept C2781338088 @default.
- W2770296654 hasConcept C37263863 @default.
- W2770296654 hasConcept C41183919 @default.
- W2770296654 hasConcept C515207424 @default.
- W2770296654 hasConcept C523546767 @default.
- W2770296654 hasConcept C54355233 @default.
- W2770296654 hasConcept C547475151 @default.
- W2770296654 hasConcept C55493867 @default.
- W2770296654 hasConcept C86803240 @default.
- W2770296654 hasConceptScore W2770296654C103408237 @default.
- W2770296654 hasConceptScore W2770296654C104317684 @default.