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- W2770360918 abstract "Abstract The Helicobacter pylori phase variable gene modH , typified by gene HP1522 in strain 26695, encodes a N 6 -adenosine type III DNA methyltransferase. Our previous studies identified multiple strain-specific modH variants ( modH1 – modH19 ) and showed that phase variation of modH5 in H. pylori P12 influenced expression of motility-associated genes and outer membrane protein gene hopG . However, the ModH5 DNA recognition motif and the mechanism by which ModH5 controls gene expression were unknown. Here, using comparative single molecule real-time sequencing, we identify the DNA site methylated by ModH5 as 5′-G m6 ACC-3′. This motif is vastly underrepresented in H. pylori genomes, but overrepresented in a number of virulence genes, including motility-associated genes, and outer membrane protein genes. Motility and the number of flagella of H. pylori P12 wild-type were significantly higher than that of isogenic modH5 OFF or Δ modH5 mutants, indicating that phase variable switching of modH5 expression plays a role in regulating H. pylori motility phenotypes. Using the flagellin A ( flaA ) gene as a model, we show that ModH5 modulates flaA promoter activity in a GACC methylation-dependent manner. These findings provide novel insights into the role of ModH5 in gene regulation and how it mediates epigenetic regulation of H. pylori motility." @default.
- W2770360918 created "2017-12-04" @default.
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- W2770360918 date "2017-11-23" @default.
- W2770360918 modified "2023-09-23" @default.
- W2770360918 title "Methylomic and phenotypic analysis of the ModH5 phasevarion of Helicobacter pylori" @default.
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- W2770360918 doi "https://doi.org/10.1038/s41598-017-15721-x" @default.
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