Matches in SemOpenAlex for { <https://semopenalex.org/work/W2770540507> ?p ?o ?g. }
- W2770540507 endingPage "3101" @default.
- W2770540507 startingPage "3085" @default.
- W2770540507 abstract "In prokaryotic systems, the translation initiation of many, though not all, mRNAs depends on interaction between a sequence element upstream of the start codon (the Shine-Dalgarno sequence [SD]) and a complementary sequence in the 3' end of the 16S rRNA (anti-Shine-Dalgarno sequence [aSD]). Although many chloroplast mRNAs harbor putative SDs in their 5' untranslated regions and the aSD displays strong conservation, the functional relevance of SD-aSD interactions in plastid translation is unclear. Here, by generating transplastomic tobacco (Nicotiana tabacum) mutants with point mutations in the aSD coupled with genome-wide analysis of translation by ribosome profiling, we provide a global picture of SD-dependent translation in plastids. We observed a pronounced correlation between weakened predicted SD-aSD interactions and reduced translation efficiency. However, multiple lines of evidence suggest that the strength of the SD-aSD interaction is not the only determinant of the translational output of many plastid mRNAs. Finally, the translation efficiency of mRNAs with strong secondary structures around the start codon is more dependent on the SD-aSD interaction than weakly structured mRNAs. Thus, our data reveal the importance of the aSD in plastid translation initiation, uncover chloroplast genes whose translation is influenced by SD-aSD interactions, and provide insights into determinants of translation efficiency in plastids." @default.
- W2770540507 created "2017-12-04" @default.
- W2770540507 creator A5017040043 @default.
- W2770540507 creator A5021276539 @default.
- W2770540507 creator A5024026397 @default.
- W2770540507 creator A5045713419 @default.
- W2770540507 creator A5065364326 @default.
- W2770540507 creator A5065869297 @default.
- W2770540507 creator A5075199511 @default.
- W2770540507 creator A5081417275 @default.
- W2770540507 creator A5090347411 @default.
- W2770540507 date "2017-11-13" @default.
- W2770540507 modified "2023-10-15" @default.
- W2770540507 title "Shine-Dalgarno Sequences Play an Essential Role in the Translation of Plastid mRNAs in Tobacco" @default.
- W2770540507 cites W1216179325 @default.
- W2770540507 cites W13691622 @default.
- W2770540507 cites W1510364105 @default.
- W2770540507 cites W1547440311 @default.
- W2770540507 cites W1830373619 @default.
- W2770540507 cites W1922497225 @default.
- W2770540507 cites W1968417067 @default.
- W2770540507 cites W1968830816 @default.
- W2770540507 cites W1969538062 @default.
- W2770540507 cites W1969749135 @default.
- W2770540507 cites W1971421846 @default.
- W2770540507 cites W1976431857 @default.
- W2770540507 cites W1978758498 @default.
- W2770540507 cites W1982790821 @default.
- W2770540507 cites W1983106607 @default.
- W2770540507 cites W1986078051 @default.
- W2770540507 cites W1986493208 @default.
- W2770540507 cites W1987882055 @default.
- W2770540507 cites W1989004575 @default.
- W2770540507 cites W2000219748 @default.
- W2770540507 cites W2009222089 @default.
- W2770540507 cites W2011739907 @default.
- W2770540507 cites W2013321787 @default.
- W2770540507 cites W2016718974 @default.
- W2770540507 cites W2019661196 @default.
- W2770540507 cites W2030198901 @default.
- W2770540507 cites W2035475950 @default.
- W2770540507 cites W2036352174 @default.
- W2770540507 cites W2039756922 @default.
- W2770540507 cites W2045416532 @default.
- W2770540507 cites W2051242197 @default.
- W2770540507 cites W2051612911 @default.
- W2770540507 cites W2053167380 @default.
- W2770540507 cites W2056971458 @default.
- W2770540507 cites W2058426472 @default.
- W2770540507 cites W2059660075 @default.
- W2770540507 cites W2061659241 @default.
- W2770540507 cites W2066775995 @default.
- W2770540507 cites W2067535938 @default.
- W2770540507 cites W2068167761 @default.
- W2770540507 cites W2069020117 @default.
- W2770540507 cites W2074425133 @default.
- W2770540507 cites W2075779791 @default.
- W2770540507 cites W2079517684 @default.
- W2770540507 cites W2081283635 @default.
- W2770540507 cites W2082873735 @default.
- W2770540507 cites W2083350289 @default.
- W2770540507 cites W2092035637 @default.
- W2770540507 cites W2096810136 @default.
- W2770540507 cites W2101405811 @default.
- W2770540507 cites W2103190835 @default.
- W2770540507 cites W2106535769 @default.
- W2770540507 cites W2108173458 @default.
- W2770540507 cites W2109810614 @default.
- W2770540507 cites W2114206214 @default.
- W2770540507 cites W2115188908 @default.
- W2770540507 cites W2115858076 @default.
- W2770540507 cites W2119336718 @default.
- W2770540507 cites W2120921946 @default.
- W2770540507 cites W2122490658 @default.
- W2770540507 cites W2122782466 @default.
- W2770540507 cites W2124727354 @default.
- W2770540507 cites W2126014276 @default.
- W2770540507 cites W2132824112 @default.
- W2770540507 cites W2132839856 @default.
- W2770540507 cites W2132990510 @default.
- W2770540507 cites W2133225212 @default.
- W2770540507 cites W2134860368 @default.
- W2770540507 cites W2136144179 @default.
- W2770540507 cites W2136781454 @default.
- W2770540507 cites W2141084464 @default.
- W2770540507 cites W2142857824 @default.
- W2770540507 cites W2148440285 @default.
- W2770540507 cites W2151911204 @default.
- W2770540507 cites W2152490734 @default.
- W2770540507 cites W2153276014 @default.
- W2770540507 cites W2153523478 @default.
- W2770540507 cites W2154241726 @default.
- W2770540507 cites W2154809818 @default.
- W2770540507 cites W2156320594 @default.
- W2770540507 cites W2158241205 @default.
- W2770540507 cites W2164800953 @default.
- W2770540507 cites W2166064969 @default.
- W2770540507 cites W2167905787 @default.