Matches in SemOpenAlex for { <https://semopenalex.org/work/W2770657648> ?p ?o ?g. }
- W2770657648 abstract "Polycomb repressive complex 2 (PRC2) is a key chromatin modifier responsible for methylation of lysine 27 in histone H3. PRC2 has been shown to interact with thousands of RNA species in vivo, but understanding the physiological function of RNA binding has been hampered by the lack of separation-of-function mutants. Here, we use comprehensive mutagenesis and hydrogen deuterium exchange mass spectrometry (HDX-MS) to identify critical residues for RNA interaction in PRC2 core complexes from Homo sapiens and Chaetomium thermophilum, for which crystal structures are known. Preferential binding of G-quadruplex RNA is conserved, surprisingly using different protein elements. Key RNA-binding residues are spread out along the surface of EZH2, with other subunits including EED also contributing, and missense mutations of some of these residues have been found in cancer patients. The unusual nature of this protein-RNA interaction provides a paradigm for other epigenetic modifiers that bind RNA without canonical RNA-binding motifs." @default.
- W2770657648 created "2017-12-04" @default.
- W2770657648 creator A5002423435 @default.
- W2770657648 creator A5005854473 @default.
- W2770657648 creator A5006518535 @default.
- W2770657648 creator A5011697275 @default.
- W2770657648 creator A5014095609 @default.
- W2770657648 creator A5017573996 @default.
- W2770657648 creator A5019961292 @default.
- W2770657648 creator A5021687717 @default.
- W2770657648 creator A5034292708 @default.
- W2770657648 creator A5050391600 @default.
- W2770657648 creator A5067427266 @default.
- W2770657648 creator A5071037763 @default.
- W2770657648 creator A5071409456 @default.
- W2770657648 date "2017-11-29" @default.
- W2770657648 modified "2023-10-18" @default.
- W2770657648 title "Conserved RNA-binding specificity of polycomb repressive complex 2 is achieved by dispersed amino acid patches in EZH2" @default.
- W2770657648 cites W1559605245 @default.
- W2770657648 cites W1566897994 @default.
- W2770657648 cites W1963807858 @default.
- W2770657648 cites W1968643850 @default.
- W2770657648 cites W1969578494 @default.
- W2770657648 cites W1972170164 @default.
- W2770657648 cites W1976242511 @default.
- W2770657648 cites W1976913448 @default.
- W2770657648 cites W1977943782 @default.
- W2770657648 cites W1984023556 @default.
- W2770657648 cites W2006107100 @default.
- W2770657648 cites W2010673383 @default.
- W2770657648 cites W2010790480 @default.
- W2770657648 cites W2011181841 @default.
- W2770657648 cites W2015132841 @default.
- W2770657648 cites W2016830527 @default.
- W2770657648 cites W2022508345 @default.
- W2770657648 cites W2027870104 @default.
- W2770657648 cites W2033586326 @default.
- W2770657648 cites W2046792302 @default.
- W2770657648 cites W2047909579 @default.
- W2770657648 cites W2053758959 @default.
- W2770657648 cites W2058673308 @default.
- W2770657648 cites W2059968749 @default.
- W2770657648 cites W2060608189 @default.
- W2770657648 cites W2061925865 @default.
- W2770657648 cites W2072466432 @default.
- W2770657648 cites W2075087486 @default.
- W2770657648 cites W2078616374 @default.
- W2770657648 cites W2084990854 @default.
- W2770657648 cites W2094032514 @default.
- W2770657648 cites W2103419911 @default.
- W2770657648 cites W2108078422 @default.
- W2770657648 cites W2111473969 @default.
- W2770657648 cites W2113999582 @default.
- W2770657648 cites W2114029110 @default.
- W2770657648 cites W2116236561 @default.
- W2770657648 cites W2120330019 @default.
- W2770657648 cites W2122076742 @default.
- W2770657648 cites W2132747713 @default.
- W2770657648 cites W2133308819 @default.
- W2770657648 cites W2141452262 @default.
- W2770657648 cites W2145502498 @default.
- W2770657648 cites W2149821525 @default.
- W2770657648 cites W2153424791 @default.
- W2770657648 cites W2154284135 @default.
- W2770657648 cites W2154535417 @default.
- W2770657648 cites W2163325684 @default.
- W2770657648 cites W2166164324 @default.
- W2770657648 cites W2283206150 @default.
- W2770657648 cites W2344518204 @default.
- W2770657648 cites W2345109029 @default.
- W2770657648 cites W2345850272 @default.
- W2770657648 cites W2522261353 @default.
- W2770657648 cites W2596157468 @default.
- W2770657648 cites W2760056442 @default.
- W2770657648 cites W2765753520 @default.
- W2770657648 doi "https://doi.org/10.7554/elife.31558" @default.
- W2770657648 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5706960" @default.
- W2770657648 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29185984" @default.
- W2770657648 hasPublicationYear "2017" @default.
- W2770657648 type Work @default.
- W2770657648 sameAs 2770657648 @default.
- W2770657648 citedByCount "73" @default.
- W2770657648 countsByYear W27706576482018 @default.
- W2770657648 countsByYear W27706576482019 @default.
- W2770657648 countsByYear W27706576482020 @default.
- W2770657648 countsByYear W27706576482021 @default.
- W2770657648 countsByYear W27706576482022 @default.
- W2770657648 countsByYear W27706576482023 @default.
- W2770657648 crossrefType "journal-article" @default.
- W2770657648 hasAuthorship W2770657648A5002423435 @default.
- W2770657648 hasAuthorship W2770657648A5005854473 @default.
- W2770657648 hasAuthorship W2770657648A5006518535 @default.
- W2770657648 hasAuthorship W2770657648A5011697275 @default.
- W2770657648 hasAuthorship W2770657648A5014095609 @default.
- W2770657648 hasAuthorship W2770657648A5017573996 @default.
- W2770657648 hasAuthorship W2770657648A5019961292 @default.
- W2770657648 hasAuthorship W2770657648A5021687717 @default.
- W2770657648 hasAuthorship W2770657648A5034292708 @default.
- W2770657648 hasAuthorship W2770657648A5050391600 @default.
- W2770657648 hasAuthorship W2770657648A5067427266 @default.