FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2771373031> ?p ?o ?g. }

• W2771373031 endingPage "154" @default.
• W2771373031 startingPage "137" @default.
• W2771373031 abstract "The dynamics of metal binding to and transfer from metalloproteins involved in metal homeostasis are important for understanding cellular distribution of metal ions. The dicotyledonous plant Arabidopsis thaliana has two type 4 seed-specific metallothionein homologues, MT4a and MT4b, with likely roles in zinc(II) homeostasis. These two metallothioneins are 84% identical, with full conservation of all metal-binding cysteine and histidine residues. Yet, differences in their spatial and temporal expression patterns suggested divergence in their biological roles. To investigate whether biological functions are reflected in molecular properties, we compare aspects of zinc(II)-binding dynamics of full-length MT4a and MT4b, namely the pH dependence of zinc(II) binding and protein folding, and zinc(II) transfer to the chelator EDTA. UV-Vis and NMR spectroscopies as well as native electrospray ionisation mass spectrometry consistently showed that transfer from Zn6MT4a is considerably faster than from Zn6MT4b, with pseudo-first-order rate constants for the fastest observed step of k obs = 2.8 × 10-4 s-1 (MT4b) and k obs = 7.5 × 10-4 s-1 (MT4a) (5 µM protein, 500 µM EDTA, 25 mM Tris buffer, pH 7.33, 298 K). 2D heteronuclear NMR experiments allowed locating the most labile zinc(II) ions in domain II for both proteins. 3D homology models suggest that reactivity of this domain is governed by the local environment around the mononuclear Cys2His2 site that is unique to type 4 MTs. Non-conservative amino acid substitutions in this region affect local electrostatics as well as whole-domain dynamics, with both effects rendering zinc(II) ions bound to MT4a more reactive in metal transfer reactions. Therefore, domain II of MT4a is well suited to rapidly release its bound zinc(II) ions, in broad agreement with a previously suggested role of MT4a in zinc(II) transport and delivery to other proteins." @default.
• W2771373031 created "2017-12-22" @default.
• W2771373031 creator A5028198037 @default.
• W2771373031 creator A5091310657 @default.
• W2771373031 date "2017-12-07" @default.
• W2771373031 modified "2023-09-25" @default.
• W2771373031 title "Differential reactivity of closely related zinc(II)-binding metallothioneins from the plant Arabidopsis thaliana" @default.
• W2771373031 cites W1513279392 @default.
• W2771373031 cites W1570809811 @default.
• W2771373031 cites W1607127186 @default.
• W2771373031 cites W1773592769 @default.
• W2771373031 cites W1964555204 @default.
• W2771373031 cites W1965073499 @default.
• W2771373031 cites W1967301498 @default.
• W2771373031 cites W1971691741 @default.
• W2771373031 cites W1972992920 @default.
• W2771373031 cites W1974063220 @default.
• W2771373031 cites W1975773040 @default.
• W2771373031 cites W1978510970 @default.
• W2771373031 cites W1980133924 @default.
• W2771373031 cites W1991105697 @default.
• W2771373031 cites W1991740092 @default.
• W2771373031 cites W1992240624 @default.
• W2771373031 cites W1993153443 @default.
• W2771373031 cites W1994447136 @default.
• W2771373031 cites W1995737411 @default.
• W2771373031 cites W1999331386 @default.
• W2771373031 cites W2013240224 @default.
• W2771373031 cites W2013340413 @default.
• W2771373031 cites W2015143005 @default.
• W2771373031 cites W2020168547 @default.
• W2771373031 cites W2020627040 @default.
• W2771373031 cites W2021002667 @default.
• W2771373031 cites W2024463079 @default.
• W2771373031 cites W2026620421 @default.
• W2771373031 cites W2031020196 @default.
• W2771373031 cites W2031341581 @default.
• W2771373031 cites W2039920572 @default.
• W2771373031 cites W2041631122 @default.
• W2771373031 cites W2047098048 @default.
• W2771373031 cites W2047399603 @default.
• W2771373031 cites W2049741584 @default.
• W2771373031 cites W2050551315 @default.
• W2771373031 cites W2051126279 @default.
• W2771373031 cites W2052865318 @default.
• W2771373031 cites W2054569628 @default.
• W2771373031 cites W2063829973 @default.
• W2771373031 cites W2064349216 @default.
• W2771373031 cites W2064986717 @default.
• W2771373031 cites W2065246746 @default.
• W2771373031 cites W2066659411 @default.
• W2771373031 cites W2074758034 @default.
• W2771373031 cites W2079387486 @default.
• W2771373031 cites W2080270884 @default.
• W2771373031 cites W2081846456 @default.
• W2771373031 cites W2083278094 @default.
• W2771373031 cites W2094895714 @default.
• W2771373031 cites W2097951289 @default.
• W2771373031 cites W2099600197 @default.
• W2771373031 cites W2100040663 @default.
• W2771373031 cites W2101809544 @default.
• W2771373031 cites W2102350033 @default.
• W2771373031 cites W2105338725 @default.
• W2771373031 cites W2105452080 @default.
• W2771373031 cites W2106648157 @default.
• W2771373031 cites W2106769439 @default.
• W2771373031 cites W2108849429 @default.
• W2771373031 cites W2110279436 @default.
• W2771373031 cites W2111727577 @default.
• W2771373031 cites W2113186717 @default.
• W2771373031 cites W2114055738 @default.
• W2771373031 cites W2114520383 @default.
• W2771373031 cites W2120007834 @default.
• W2771373031 cites W2123662095 @default.
• W2771373031 cites W2124960654 @default.
• W2771373031 cites W2125181974 @default.
• W2771373031 cites W2132717858 @default.
• W2771373031 cites W2135731574 @default.
• W2771373031 cites W2141969709 @default.
• W2771373031 cites W2147085096 @default.
• W2771373031 cites W2147702594 @default.
• W2771373031 cites W2150144448 @default.
• W2771373031 cites W2150163895 @default.
• W2771373031 cites W2163135980 @default.
• W2771373031 cites W2163208441 @default.
• W2771373031 cites W2198508198 @default.
• W2771373031 cites W2206352696 @default.
• W2771373031 cites W2212963707 @default.
• W2771373031 cites W2231134786 @default.
• W2771373031 cites W2260945830 @default.
• W2771373031 cites W2264552914 @default.
• W2771373031 cites W2268731531 @default.
• W2771373031 cites W2293779074 @default.
• W2771373031 cites W2295616246 @default.
• W2771373031 cites W2316391400 @default.
• W2771373031 cites W2319069564 @default.
• W2771373031 cites W2320930590 @default.
• W2771373031 cites W2327522222 @default.

• next