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- W2771787069 abstract "Abstract Caenorhabditis elegans are typically cultured in a monoxenic medium consisting of live bacteria. However, this introduces a secondary organism to experiments, and restricts the manipulation of the nutritional environment. Due to the intricate link between genes and environment, greater control and understanding of nutritional factors are required to push the C. elegans field into new areas. For decades, attempts to develop a chemically defined, axenic medium as an alternative for culturing C. elegans have been made. However, the mechanism by which the filter feeder C. elegans obtains nutrients from these liquid media is not known. Using a fluorescence-activated cell sorting based approach, we demonstrate growth in all past axenic C. elegans media to be dependent on the presence of previously unknown particles. This particle requirement of C. elegans led to development of liposome-based, nanoparticle culturing that allows full control of nutrients delivered to C. elegans." @default.
- W2771787069 created "2017-12-22" @default.
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- W2771787069 date "2018-02-01" @default.
- W2771787069 modified "2023-10-10" @default.
- W2771787069 title "Growth of<i>Caenorhabditis elegans</i>in Defined Media Is Dependent on Presence of Particulate Matter" @default.
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- W2771787069 doi "https://doi.org/10.1534/g3.117.300325" @default.
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