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- W2772022205 abstract "The aim of this investigation was to evaluate the efficiency of supplementing ram semen extender with melatonin on cryopreservation capacity of spermatozoa. A total of 80 ejaculates were collected from 5 Barki rams, 16 ejaculates each, by an artificial vagina throughout the period from January – February, 2017. Ejaculates of each collection session were pooled and diluted (1:10) with glycerolated Tris-citric egg yolk extender, and were split into 4 portions. The first portion served as control (melatonin-free), whereas the other 3 portions were supplemented with 0.1, 0.2 or 0.3 mM melatonin. The post-thaw objective assessment of cryopreserved spermatozoa, in all groups, was conducted by a computer-assisted sperm analysis (CASA) system. The results revealed that melatonin supplementation was positively correlated (P<0.01) with post-thaw total motility, progressive motility, viability, acrosome integrity, sperm cell membrane integrity, VSL, VAP and WOB (r= 0.88, 0.71, 0.78, 0.85, 0.96, 0.95, 0.68, 0.57 and 0.53, respectively. However, it was negatively correlated (P<0.01) with primary and secondary sperm abnormalities, as well as non-progressive motility and immotile spermatozoa (r= -0.73, -0.85, -0.49 and -0.76, respectively. These results elucidate that adding melatonin to ram semen extender substantially enhanced post-thaw sperm physical properties, which implies its powerful potential as an exogenous antioxidant supplement." @default.
- W2772022205 created "2017-12-22" @default.
- W2772022205 creator A5061429346 @default.
- W2772022205 date "2017-01-01" @default.
- W2772022205 modified "2023-10-18" @default.
- W2772022205 title "Improving cryopreservation capacity of ram spermatozoa by supplementing the diluent with melatonin" @default.
- W2772022205 doi "https://doi.org/10.28933/ijar-2017-11-0202" @default.
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