FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2772035889> ?p ?o ?g. }

• W2772035889 endingPage "2610" @default.
• W2772035889 startingPage "2610" @default.
• W2772035889 abstract "The recent advancement in genome editing such a CRISPR/Cas9 system has enabled isolation of cells with knocked multiple alleles through a one-step transfection. Somatic cell nuclear transfer (SCNT) has been frequently employed as one of the efficient tools for the production of genetically modified (GM) animals. To use GM cells as SCNT donor, efficient isolation of transfectants with mutations at multiple target loci is often required. The methods for the isolation of such GM cells largely rely on the use of drug selection-based approach using selectable genes; however, it is often difficult to isolate cells with mutations at multiple target loci. In this study, we used a novel approach for the efficient isolation of porcine cells with at least two target loci mutations by one-step introduction of CRISPR/Cas9-related components. A single guide (sg) RNA targeted to GGTA1 gene, involved in the synthesis of cell-surface α-Gal epitope (known as xenogenic antigen), is always a prerequisite. When the transfected cells were reacted with toxin-labeled BS-I-B4 isolectin for 2 h at 37 °C to eliminate α-Gal epitope-expressing cells, the surviving clones lacked α-Gal epitope expression and were highly expected to exhibit induced mutations at another target loci. Analysis of these α-Gal epitope-negative surviving cells demonstrated a 100% occurrence of genome editing at target loci. SCNT using these cells as donors resulted in the production of cloned blastocysts with the genotype similar to that of the donor cells used. Thus, this novel system will be useful for SCNT-mediated acquisition of GM cloned piglets, in which multiple target loci may be mutated." @default.
• W2772035889 created "2017-12-22" @default.
• W2772035889 creator A5013460644 @default.
• W2772035889 creator A5037206421 @default.
• W2772035889 creator A5037289392 @default.
• W2772035889 creator A5037769750 @default.
• W2772035889 creator A5046004509 @default.
• W2772035889 creator A5055981066 @default.
• W2772035889 creator A5058231632 @default.
• W2772035889 creator A5075765457 @default.
• W2772035889 date "2017-12-04" @default.
• W2772035889 modified "2023-10-16" @default.
• W2772035889 title "Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System" @default.
• W2772035889 cites W1528466007 @default.
• W2772035889 cites W1544032942 @default.
• W2772035889 cites W1845305428 @default.
• W2772035889 cites W1910916458 @default.
• W2772035889 cites W1977047711 @default.
• W2772035889 cites W1977709885 @default.
• W2772035889 cites W1978984490 @default.
• W2772035889 cites W1983908831 @default.
• W2772035889 cites W1984508363 @default.
• W2772035889 cites W1987909360 @default.
• W2772035889 cites W1989605407 @default.
• W2772035889 cites W1989606549 @default.
• W2772035889 cites W1992524499 @default.
• W2772035889 cites W1996186364 @default.
• W2772035889 cites W1997509018 @default.
• W2772035889 cites W2003171404 @default.
• W2772035889 cites W2004468708 @default.
• W2772035889 cites W2011012950 @default.
• W2772035889 cites W2026351661 @default.
• W2772035889 cites W2036176224 @default.
• W2772035889 cites W2036659145 @default.
• W2772035889 cites W2036779682 @default.
• W2772035889 cites W2038553774 @default.
• W2772035889 cites W2061403184 @default.
• W2772035889 cites W2064765186 @default.
• W2772035889 cites W2064815984 @default.
• W2772035889 cites W2072070315 @default.
• W2772035889 cites W2077659966 @default.
• W2772035889 cites W2078835422 @default.
• W2772035889 cites W2078863744 @default.
• W2772035889 cites W2086463825 @default.
• W2772035889 cites W2086892188 @default.
• W2772035889 cites W2091894237 @default.
• W2772035889 cites W2110553915 @default.
• W2772035889 cites W2133785873 @default.
• W2772035889 cites W2134002612 @default.
• W2772035889 cites W2146092834 @default.
• W2772035889 cites W2149402050 @default.
• W2772035889 cites W2150273306 @default.
• W2772035889 cites W2154253440 @default.
• W2772035889 cites W2157361557 @default.
• W2772035889 cites W2161810908 @default.
• W2772035889 cites W2163649189 @default.
• W2772035889 cites W2163780906 @default.
• W2772035889 cites W2164098707 @default.
• W2772035889 cites W2167171238 @default.
• W2772035889 cites W2308010778 @default.
• W2772035889 cites W2414056476 @default.
• W2772035889 cites W2462175589 @default.
• W2772035889 cites W2553373918 @default.
• W2772035889 cites W2591499037 @default.
• W2772035889 cites W2003346177 @default.
• W2772035889 doi "https://doi.org/10.3390/ijms18122610" @default.
• W2772035889 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5751213" @default.
• W2772035889 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29207527" @default.
• W2772035889 hasPublicationYear "2017" @default.
• W2772035889 type Work @default.
• W2772035889 sameAs 2772035889 @default.
• W2772035889 citedByCount "7" @default.
• W2772035889 countsByYear W27720358892018 @default.
• W2772035889 countsByYear W27720358892019 @default.
• W2772035889 countsByYear W27720358892020 @default.
• W2772035889 crossrefType "journal-article" @default.
• W2772035889 hasAuthorship W2772035889A5013460644 @default.
• W2772035889 hasAuthorship W2772035889A5037206421 @default.
• W2772035889 hasAuthorship W2772035889A5037289392 @default.
• W2772035889 hasAuthorship W2772035889A5037769750 @default.
• W2772035889 hasAuthorship W2772035889A5046004509 @default.
• W2772035889 hasAuthorship W2772035889A5055981066 @default.
• W2772035889 hasAuthorship W2772035889A5058231632 @default.
• W2772035889 hasAuthorship W2772035889A5075765457 @default.
• W2772035889 hasBestOaLocation W27720358891 @default.
• W2772035889 hasConcept C104317684 @default.
• W2772035889 hasConcept C111829913 @default.
• W2772035889 hasConcept C132455925 @default.
• W2772035889 hasConcept C134305767 @default.
• W2772035889 hasConcept C144501496 @default.
• W2772035889 hasConcept C147483822 @default.
• W2772035889 hasConcept C153911025 @default.
• W2772035889 hasConcept C195616568 @default.
• W2772035889 hasConcept C202438428 @default.
• W2772035889 hasConcept C22744801 @default.
• W2772035889 hasConcept C2778177303 @default.
• W2772035889 hasConcept C42411736 @default.
• W2772035889 hasConcept C54009773 @default.

• next