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• W2772037567 startingPage "153601211880958" @default.
• W2772037567 abstract "Metastasis is the most common cause of death for patients with cancer. To fully understand the steps involved in metastatic dissemination, in vivo models are required, of which murine ones are the most common. Therefore, preclinical imaging methods such as magnetic resonance imaging (MRI) have mainly been developed for small mammals and their potential to monitor cancer growth and metastasis in nonmammalian models is not fully harnessed. We have here used MRI to measure primary neuroblastoma tumor size and metastasis in a chick embryo model. We compared its sensitivity and accuracy to end-point fluorescence detection upon dissection. Human neuroblastoma cells labeled with green fluorescent protein (GFP) and micron-sized iron particles were implanted on the extraembryonic chorioallantoic membrane of the chick at E7. T 2 RARE, T 2 -weighted fast low angle shot (FLASH) as well as time-of-flight MR angiography imaging were applied at E14. Micron-sized iron particle labeling of neuroblastoma cells allowed in ovo observation of the primary tumor and tumor volume measurement noninvasively. Moreover, T 2 weighted and FLASH imaging permitted the detection of small metastatic deposits in the chick embryo, thereby reinforcing the potential of this convenient, 3R compliant, in vivo model for cancer research." @default.
• W2772037567 created "2017-12-22" @default.
• W2772037567 creator A5006267936 @default.
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• W2772037567 date "2018-01-01" @default.
• W2772037567 modified "2023-10-16" @default.
• W2772037567 title "Magnetic Resonance Imaging for Characterization of a Chick Embryo Model of Cancer Cell Metastases" @default.
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• W2772037567 doi "https://doi.org/10.1177/1536012118809585" @default.
• W2772037567 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6236852" @default.
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• W2772037567 hasPublicationYear "2018" @default.
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