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• W2772079366 abstract "We investigate the use of polarized illumination in multiview microscopes for determining the orientation of single-molecule fluorescence transition dipoles. First, we relate the orientation of single dipoles to measurable intensities in multiview microscopes and develop an information-theoretic metric-the solid-angle uncertainty-to compare the ability of multiview microscopes to estimate the orientation of single dipoles. Next, we compare a broad class of microscopes using this metric-single- and dual-view microscopes with varying illumination polarization, illumination numerical aperture (NA), detection NA, obliquity, asymmetry, and exposure. We find that multi-view microscopes can measure all dipole orientations, while the orientations measurable with single-view microscopes is halved because of symmetries in the detection process. We also find that choosing a small illumination NA and a large detection NA are good design choices, that multiview microscopes can benefit from oblique illumination and detection, and that asymmetric NA microscopes can benefit from exposure asymmetry." @default.
• W2772079366 created "2017-12-22" @default.
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• W2772079366 date "2017-12-01" @default.
• W2772079366 modified "2023-10-17" @default.
• W2772079366 title "Single-fluorophore orientation determination with multiview polarized illumination: modeling and microscope design" @default.
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• W2772079366 doi "https://doi.org/10.1364/oe.25.031309" @default.
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