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- W2772299044 abstract "We have recently developed a photolabeling method to identify GTP-loaded Rab proteins. The new biotinylated GTP analogue (Bio-ATB-GTP) binds to GTP-binding proteins and after a UV irradiation a covalent bond is formed between the protein and the photoreactive diazirine group on the photolabel. The tagged protein can then be isolated and detected using the classic biotin-streptavidin interaction. In this chapter, we describe the Bio-ATB-GTP photolabel and discuss the advantages of using this photolabeling approach to detect GTP-loaded Rab proteins compared to other existing methodologies. We also describe a step-by-step procedure for detecting the activated state of a Rab protein in primary rat adipocytes." @default.
- W2772299044 created "2017-12-22" @default.
- W2772299044 creator A5024552542 @default.
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- W2772299044 date "2017-12-08" @default.
- W2772299044 modified "2023-09-27" @default.
- W2772299044 title "Identification of Insulin-Activated Rab Proteins in Adipose Cells Using Bio-ATB-GTP Photolabeling Technique" @default.
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- W2772299044 doi "https://doi.org/10.1007/978-1-4939-7507-5_11" @default.
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