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- W2772374884 abstract "Western blotting method to detect thiophosphorylated Src. Src (catalytic domain, 295 nM) was incubated alone (unphos), with 1 mM ATP and 5 mM MgCl2 (phos), or with 1 mM ATPγS and 10 mM NiCl2 (thiophos) for 30 min at 30 °C. The reactions were analyzed by SDS-PAGE and Western blotting with anti-pTyr antibody (top) and with anti-Src (pY416) antibody (bottom). In the right-hand lane of the gel, four times the amount of Src was loaded as in the other lanes. (PDF 466 kb)" @default.
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- W2772374884 date "2016-01-01" @default.
- W2772374884 modified "2023-09-22" @default.
- W2772374884 title "Additional file 3: Figure S3. of Auto-thiophosphorylation activity of Src tyrosine kinase" @default.
- W2772374884 doi "https://doi.org/10.6084/m9.figshare.c.3631793_d1" @default.
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