Matches in SemOpenAlex for { <https://semopenalex.org/work/W2772578804> ?p ?o ?g. }
- W2772578804 endingPage "609" @default.
- W2772578804 startingPage "600" @default.
- W2772578804 abstract "In this study, a single-domain antibody against negative regulatory factor (anti-NEF scFv) was autodisplayed on the outer membrane of Escherichia coli and used to detect NEF in an immunoassay based on fluorescence-activated cell sorting, enzyme-linked immunosorbent assay, and surface plasmon resonance biosensors. Next, the autodisplayed single-domain antibody was oxidized to form disulfide bonds by using glutathione, and the change in NEF-binding activity of anti-NEF scFv was analyzed by fluorescence-activated cell sorting-based immunoassay, chromogenic immunoassay, and surface plasmon resonance biosensor. For each type of immunoassays the anti-NEF scFv on the isolated outer membrane showed more NEF binding activity after the disulfide bond formation by glutathione. To determine the role of cysteines in anti-NEF scFv, three mutants were prepared, and the NEF binding activity of mutants was compared with that of wild-type anti-NEF scFv in a competitive immunoassay based on FACS. In these mutant studies, the refolding process of autodisplayed anti-NEF scFv by following oxidation via GSH/GSSG revealed that disulfide bonds formed and increased NEF binding activity." @default.
- W2772578804 created "2017-12-22" @default.
- W2772578804 creator A5014892459 @default.
- W2772578804 creator A5034732409 @default.
- W2772578804 creator A5039960807 @default.
- W2772578804 creator A5057664407 @default.
- W2772578804 creator A5074944925 @default.
- W2772578804 creator A5081645354 @default.
- W2772578804 date "2018-04-01" @default.
- W2772578804 modified "2023-10-16" @default.
- W2772578804 title "Refolding of autodisplayed anti-NEF scFv through oxidation with glutathione for immunosensors" @default.
- W2772578804 cites W1733847523 @default.
- W2772578804 cites W1991622878 @default.
- W2772578804 cites W1999010436 @default.
- W2772578804 cites W2005133991 @default.
- W2772578804 cites W2005726038 @default.
- W2772578804 cites W2011941526 @default.
- W2772578804 cites W2015396130 @default.
- W2772578804 cites W2018862317 @default.
- W2772578804 cites W2019720327 @default.
- W2772578804 cites W2020257950 @default.
- W2772578804 cites W2021826893 @default.
- W2772578804 cites W2024492407 @default.
- W2772578804 cites W2025726959 @default.
- W2772578804 cites W2028104641 @default.
- W2772578804 cites W2033831822 @default.
- W2772578804 cites W2054550884 @default.
- W2772578804 cites W2059982100 @default.
- W2772578804 cites W2061683515 @default.
- W2772578804 cites W2072948213 @default.
- W2772578804 cites W2083247248 @default.
- W2772578804 cites W2084563847 @default.
- W2772578804 cites W2087579091 @default.
- W2772578804 cites W2090383712 @default.
- W2772578804 cites W2098356966 @default.
- W2772578804 cites W2114977781 @default.
- W2772578804 cites W2151501755 @default.
- W2772578804 cites W2151737415 @default.
- W2772578804 cites W2159614853 @default.
- W2772578804 cites W2226275287 @default.
- W2772578804 cites W2251363482 @default.
- W2772578804 cites W2344071726 @default.
- W2772578804 cites W2410057038 @default.
- W2772578804 cites W2528533631 @default.
- W2772578804 cites W2582086770 @default.
- W2772578804 cites W2592093297 @default.
- W2772578804 cites W2601363671 @default.
- W2772578804 cites W4249514799 @default.
- W2772578804 doi "https://doi.org/10.1016/j.bios.2017.12.009" @default.
- W2772578804 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29247971" @default.
- W2772578804 hasPublicationYear "2018" @default.
- W2772578804 type Work @default.
- W2772578804 sameAs 2772578804 @default.
- W2772578804 citedByCount "6" @default.
- W2772578804 countsByYear W27725788042019 @default.
- W2772578804 countsByYear W27725788042021 @default.
- W2772578804 countsByYear W27725788042022 @default.
- W2772578804 countsByYear W27725788042023 @default.
- W2772578804 crossrefType "journal-article" @default.
- W2772578804 hasAuthorship W2772578804A5014892459 @default.
- W2772578804 hasAuthorship W2772578804A5034732409 @default.
- W2772578804 hasAuthorship W2772578804A5039960807 @default.
- W2772578804 hasAuthorship W2772578804A5057664407 @default.
- W2772578804 hasAuthorship W2772578804A5074944925 @default.
- W2772578804 hasAuthorship W2772578804A5081645354 @default.
- W2772578804 hasConcept C104317684 @default.
- W2772578804 hasConcept C106847996 @default.
- W2772578804 hasConcept C143065580 @default.
- W2772578804 hasConcept C1491633281 @default.
- W2772578804 hasConcept C153911025 @default.
- W2772578804 hasConcept C155672457 @default.
- W2772578804 hasConcept C159654299 @default.
- W2772578804 hasConcept C160756335 @default.
- W2772578804 hasConcept C171250308 @default.
- W2772578804 hasConcept C181199279 @default.
- W2772578804 hasConcept C185592680 @default.
- W2772578804 hasConcept C192562407 @default.
- W2772578804 hasConcept C203014093 @default.
- W2772578804 hasConcept C2778630268 @default.
- W2772578804 hasConcept C2780420688 @default.
- W2772578804 hasConcept C538909803 @default.
- W2772578804 hasConcept C547475151 @default.
- W2772578804 hasConcept C55493867 @default.
- W2772578804 hasConcept C86803240 @default.
- W2772578804 hasConceptScore W2772578804C104317684 @default.
- W2772578804 hasConceptScore W2772578804C106847996 @default.
- W2772578804 hasConceptScore W2772578804C143065580 @default.
- W2772578804 hasConceptScore W2772578804C1491633281 @default.
- W2772578804 hasConceptScore W2772578804C153911025 @default.
- W2772578804 hasConceptScore W2772578804C155672457 @default.
- W2772578804 hasConceptScore W2772578804C159654299 @default.
- W2772578804 hasConceptScore W2772578804C160756335 @default.
- W2772578804 hasConceptScore W2772578804C171250308 @default.
- W2772578804 hasConceptScore W2772578804C181199279 @default.
- W2772578804 hasConceptScore W2772578804C185592680 @default.
- W2772578804 hasConceptScore W2772578804C192562407 @default.
- W2772578804 hasConceptScore W2772578804C203014093 @default.
- W2772578804 hasConceptScore W2772578804C2778630268 @default.