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- W2772817177 abstract "In this study, poly(-caprolactone) (PCL) was synthesized using the -caprolactone (CL) monomer ring-opening polymerization. We demonstrated that the hemoglobin (Hb) entrapped in PCL film could retain its original conformation by FT-IR spectra. A pair of well-defined redox peaks with a formal potential (E 0� ) of about −0.38 V (vs. SCE) in a pH 7.0 phosphate buffer solution was obtained at the Hb–PCL film modified GC electrode. The dependence of E 0 � on the pH of the buffer solution indicated that the conversion of heme Fe(III)/Fe(II) was a reaction of one electron coupled to one proton. The apparent heterogeneous electron transfer rate constants (ks) of Hb confined to PCL was evaluated as (18.7 ± 0.8) s −1 according to Laviron’s equation. The surface concentration (Γ * ) of the electroactive Hb in the PCL film was estimated to be (7.27 ± 0.57) × 10 −11 mol cm −2 . The Hb–PCL film modified electrode was shown to be an excellent amperometric sensor for the detection of hydrogen peroxide. The linear range is from 2 to 30M with a detection limit of 6.07 × 10 −6 M. The sensor was effectively testified by the determination of the hydrogen peroxide in eyedrops as real samples. © 2008 Elsevier B.V. All rights reserved." @default.
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- W2772817177 date "2008-01-01" @default.
- W2772817177 modified "2023-09-27" @default.
- W2772817177 title "Short communication An amperometric biosensor based on hemoglobin immobilized in poly(-caprolactone) film and its application" @default.
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- W2772817177 hasPublicationYear "2008" @default.
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