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• W2773006914 abstract "Xenorhabdus innexi is a bacterial symbiont of Steinernema scapterisci nematodes, which is a cricket-specialist parasite and together the nematode and bacteria infect and kill crickets. Curiously, X. innexi expresses a potent extracellular mosquitocidal toxin activity in culture supernatants. We sequenced a draft genome of X. innexi and compared it to the genomes of related pathogens to elucidate the nature of specialization. Using green fluorescent protein-expressing X. innexi we confirm previous reports using culture-dependent techniques that X. innexi colonizes its nematode host at low levels (~3–8 cells per nematode), relative to other Xenorhabdus-Steinernema associations. We found that compared to the well-characterized entomopathogenic nematode symbiont X. nematophila, X. innexi fails to suppress the insect phenoloxidase immune pathway and is attenuated for virulence and reproduction in the Lepidoptera Galleria mellonella and Manduca sexta, as well as the dipteran Drosophila melanogaster. To assess if, compared to other Xenorhabdus spp., X. innexi has a reduced capacity to synthesize virulence determinants, we obtained and analyzed a draft genome sequence. We found no evidence for several hallmarks of Xenorhabdus spp. toxicity, including Tc and Mcf toxins. Similar to other Xenorhabdus genomes, we found numerous loci predicted to encode non-ribosomal peptide/polyketide synthetases. Anti-SMASH predictions of these loci revealed one, related to the fcl locus that encodes fabclavines and zmn locus that encodes zeamines, as a likely candidate to encode the X. innexi mosquitocidal toxin biosynthetic machinery, which we designated Xlt. In support of this hypothesis, two mutants each with an insertion in an Xlt biosynthesis gene cluster lacked the mosquitocidal compound based on HPLC/MS analysis and neither produced toxin to the levels of the wild type parent. The X. innexi genome will be a valuable resource in identifying loci encoding new metabolites of interest, but also in future comparative studies of nematode-bacterial symbiosis and niche partitioning among bacterial pathogens." @default.
• W2773006914 created "2017-12-22" @default.
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• W2773006914 date "2017-12-01" @default.
• W2773006914 modified "2023-10-18" @default.
• W2773006914 title "The insect pathogenic bacterium Xenorhabdus innexi has attenuated virulence in multiple insect model hosts yet encodes a potent mosquitocidal toxin" @default.
• W2773006914 cites W10080646 @default.
• W2773006914 cites W1544967679 @default.
• W2773006914 cites W1546278851 @default.
• W2773006914 cites W1558645513 @default.
• W2773006914 cites W1595438182 @default.
• W2773006914 cites W179156134 @default.
• W2773006914 cites W1804054838 @default.
• W2773006914 cites W1899329792 @default.
• W2773006914 cites W1902745415 @default.
• W2773006914 cites W1908893009 @default.
• W2773006914 cites W1919814215 @default.
• W2773006914 cites W1963957860 @default.
• W2773006914 cites W1964812851 @default.
• W2773006914 cites W1966493554 @default.
• W2773006914 cites W1966688540 @default.
• W2773006914 cites W1971636065 @default.
• W2773006914 cites W1972721791 @default.
• W2773006914 cites W1973120292 @default.
• W2773006914 cites W1975221762 @default.
• W2773006914 cites W1975900720 @default.
• W2773006914 cites W1977453241 @default.
• W2773006914 cites W1979510066 @default.
• W2773006914 cites W1980518274 @default.
• W2773006914 cites W1985908083 @default.
• W2773006914 cites W1989178794 @default.
• W2773006914 cites W1993287556 @default.
• W2773006914 cites W1993774913 @default.
• W2773006914 cites W1994938388 @default.
• W2773006914 cites W2001466227 @default.
• W2773006914 cites W2004379995 @default.
• W2773006914 cites W2005102671 @default.
• W2773006914 cites W2007279356 @default.
• W2773006914 cites W2007740495 @default.
• W2773006914 cites W2009604662 @default.
• W2773006914 cites W2010486380 @default.
• W2773006914 cites W2014763250 @default.
• W2773006914 cites W2017482741 @default.
• W2773006914 cites W2025998658 @default.
• W2773006914 cites W2027322754 @default.
• W2773006914 cites W2027438561 @default.
• W2773006914 cites W2027601091 @default.
• W2773006914 cites W2028153352 @default.
• W2773006914 cites W2033342020 @default.
• W2773006914 cites W2035689050 @default.
• W2773006914 cites W2037905788 @default.
• W2773006914 cites W2039410130 @default.
• W2773006914 cites W2041262546 @default.
• W2773006914 cites W2044756191 @default.
• W2773006914 cites W2050690244 @default.
• W2773006914 cites W2051031814 @default.
• W2773006914 cites W2052709029 @default.
• W2773006914 cites W2053808754 @default.
• W2773006914 cites W2054712626 @default.
• W2773006914 cites W2055643667 @default.
• W2773006914 cites W2057294829 @default.
• W2773006914 cites W2061361921 @default.
• W2773006914 cites W2067251681 @default.
• W2773006914 cites W2070949613 @default.
• W2773006914 cites W2074966924 @default.
• W2773006914 cites W2077033667 @default.
• W2773006914 cites W2081639725 @default.
• W2773006914 cites W2083547502 @default.
• W2773006914 cites W2086460524 @default.
• W2773006914 cites W2088226054 @default.
• W2773006914 cites W2088956441 @default.
• W2773006914 cites W2090225697 @default.
• W2773006914 cites W2092490323 @default.
• W2773006914 cites W2095929632 @default.
• W2773006914 cites W2100130339 @default.
• W2773006914 cites W2100325336 @default.
• W2773006914 cites W2101014533 @default.
• W2773006914 cites W2106575068 @default.
• W2773006914 cites W2112313101 @default.
• W2773006914 cites W2112834339 @default.
• W2773006914 cites W2120635832 @default.
• W2773006914 cites W2121297944 @default.
• W2773006914 cites W2122095711 @default.
• W2773006914 cites W2125702308 @default.
• W2773006914 cites W2126072222 @default.
• W2773006914 cites W2131887397 @default.
• W2773006914 cites W2132103948 @default.

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