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- W2773048259 abstract "Background: Platelets play an important role in the formation of vascular lesions. The aim of this study was to investigate the interaction of isolated human platelets (hPlts) with human endothelium in a Rag2-/-γc-/- mouse model of human arterial xenotransplantation. Methods: Inactivated and TRAP6-stimulated hPlts were phenotyped for surface markers (CD62p,CD63) by flow cytometry. Recovery of 2'-7'-dichlorofluorescein-labeled hPlts was detected with an in vivo fluorescence imager and by flow cytometry. Side branches of human mammary artery were implanted into the infrarenal aorta of Rag2-/-γc-/- recipients, followed by daily application of 4 x 108 inactivated or activated hPlts. Arterial grafts were analyzed by histology on day 30 after transplantation. Results: Inactivated hPlts showed low levels of CD62p and CD63 [17.32 ± 2.20% / 3.75 ± 0.84% (n=6)]. After TRAP6 stimulation (activated hPlts), expression of CD62p and CD63 was markedly increased [89.03 ± 3.70% / 79.79 ± 4.13% (n=6, p<0.001)]. Circulating DCF-labeled hPlts were detected within the lung, liver, kidney, spleen and arteries of recipients. Activated platelets had lower in vivo recoveries compared to inactivated platelets after 60min [4.53 ± 0.32% / 36.71 ± 3.04% (n=5, p<0.001)]. Daily intravenous injection of inactivated or activated hPlts both groups showed intimal proliferation 30 days after transplantation [Rag2-/-γc-/- + inactivated hPlts: 59.37 ± 4.91% (n=5, p<0.001 vs. control)and Rag2-/-γc-/- + activated hPlts: 70.42 ± 19.55%(n=3)]. Conclusion:Here we can show that isolated application of inactivated or activated hPlts in the absence of T-, B- and NK-cells results in the significant amounts of transplant arteriosclerosis." @default.
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- W2773048259 date "2014-07-01" @default.
- W2773048259 modified "2023-09-23" @default.
- W2773048259 title "Isolated Transfer of Human Platelets Results in Formation of Transplant Arteriosclerosis in a RAG2-/-γ-CHAIN-/- Mouse Aortic Xenograft Model." @default.
- W2773048259 doi "https://doi.org/10.1097/00007890-201407151-01046" @default.
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