FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2773648825> ?p ?o ?g. }

• W2773648825 endingPage "155" @default.
• W2773648825 startingPage "147" @default.
• W2773648825 abstract "The complexity of ingredients in traditional Chinese medical formulas and the limited consideration of toxicological responses are fundamental issues that hamper prognostic information of drug quality control.A multidisciplinary approach for quality control of Qingkailing injection (QKL) regarding drug induced liver toxicity was described for the first time. High content image analysis (HCA) was combined with reverse-phase chromatographic separation and high-resolution MS detection technologies to provide the dynamic responses of drug induced HepG2 cell injury. Firstly, a simple and rapid method for simultaneous qualification and quantification of 21 major constituents in QKL was established and validated using ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometer (UHPLC-Q-Orbitrap), which were operated in full MS/dd-MS2 mode and thus simultaneously acquired quantitative high resolution (HR) full scan data and confirmatory HR MS2 data. Secondly, repeated semi-preparation HPLC was applied to obtain four fractions (F1-F4) for HCS analysis. Finally, potential hepatotoxicity was determined by five hepatotoxicity biomarkers, including cell loss, DNA condense, glutathione (GSH) depletion, reactive oxygen species (ROS) formation, and mitochondria membrane potential (MMP) depolarization.The detection in polarity switching mode empowered the coverage of comprehensive constituents with different chemical properties. Satisfactory linearity precisions, repeatability, stability, and recovery were achieved. QKL injection significantly induced HepG2 cell injury above the concentration of 1.25% (v/v). Meanwhile, flavone glycosides (F3) and stinasterols (F4) fractions exhibited hepatotoxicity above 75μg/mL and 50μg/mL, respectively. Still further, baicalin originated from F3 significantly caused cell loss and glutathione (GSH) depletion. In parallel, hyodeoxycholic acid from F4 induced cell loss, nucleus condense, and GSH reduction as well.Our work provides multiple perspectives based on injection-fractions-single compound format to improve QKL pharmacovigilance through revealing the potential hepatotoxic material basis. Additionally, our study provides an integrating paradigm for the comprehensive and systematic quality control of traditional Chinese medical formulas." @default.
• W2773648825 created "2017-12-22" @default.
• W2773648825 creator A5001097432 @default.
• W2773648825 creator A5003934555 @default.
• W2773648825 creator A5010951409 @default.
• W2773648825 creator A5014844124 @default.
• W2773648825 creator A5024741363 @default.
• W2773648825 creator A5031728975 @default.
• W2773648825 creator A5035381595 @default.
• W2773648825 creator A5039854550 @default.
• W2773648825 creator A5086232250 @default.
• W2773648825 date "2018-04-01" @default.
• W2773648825 modified "2023-10-10" @default.
• W2773648825 title "Multiple perspectives of qingkailing injection-fraction-single compound in revealing the hepatotoxicity of baicalin and hyodeoxycholic acid" @default.
• W2773648825 cites W1629121594 @default.
• W2773648825 cites W1779118840 @default.
• W2773648825 cites W1860370624 @default.
• W2773648825 cites W1870392458 @default.
• W2773648825 cites W1969157605 @default.
• W2773648825 cites W1990399395 @default.
• W2773648825 cites W1995429872 @default.
• W2773648825 cites W2012840245 @default.
• W2773648825 cites W2016951160 @default.
• W2773648825 cites W2020062929 @default.
• W2773648825 cites W2041024416 @default.
• W2773648825 cites W2041987241 @default.
• W2773648825 cites W2042371919 @default.
• W2773648825 cites W2075455619 @default.
• W2773648825 cites W2078291117 @default.
• W2773648825 cites W2081706995 @default.
• W2773648825 cites W2087444458 @default.
• W2773648825 cites W2095001799 @default.
• W2773648825 cites W2123435871 @default.
• W2773648825 cites W2136567948 @default.
• W2773648825 cites W2157160726 @default.
• W2773648825 cites W2184660921 @default.
• W2773648825 doi "https://doi.org/10.1016/j.jep.2017.11.035" @default.
• W2773648825 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29222033" @default.
• W2773648825 hasPublicationYear "2018" @default.
• W2773648825 type Work @default.
• W2773648825 sameAs 2773648825 @default.
• W2773648825 citedByCount "4" @default.
• W2773648825 countsByYear W27736488252018 @default.
• W2773648825 countsByYear W27736488252021 @default.
• W2773648825 countsByYear W27736488252022 @default.
• W2773648825 crossrefType "journal-article" @default.
• W2773648825 hasAuthorship W2773648825A5001097432 @default.
• W2773648825 hasAuthorship W2773648825A5003934555 @default.
• W2773648825 hasAuthorship W2773648825A5010951409 @default.
• W2773648825 hasAuthorship W2773648825A5014844124 @default.
• W2773648825 hasAuthorship W2773648825A5024741363 @default.
• W2773648825 hasAuthorship W2773648825A5031728975 @default.
• W2773648825 hasAuthorship W2773648825A5035381595 @default.
• W2773648825 hasAuthorship W2773648825A5039854550 @default.
• W2773648825 hasAuthorship W2773648825A5086232250 @default.
• W2773648825 hasConcept C154020017 @default.
• W2773648825 hasConcept C162356407 @default.
• W2773648825 hasConcept C179998833 @default.
• W2773648825 hasConcept C181199279 @default.
• W2773648825 hasConcept C185592680 @default.
• W2773648825 hasConcept C2780113650 @default.
• W2773648825 hasConcept C2780841973 @default.
• W2773648825 hasConcept C43617362 @default.
• W2773648825 hasConcept C538909803 @default.
• W2773648825 hasConcept C55493867 @default.
• W2773648825 hasConceptScore W2773648825C154020017 @default.
• W2773648825 hasConceptScore W2773648825C162356407 @default.
• W2773648825 hasConceptScore W2773648825C179998833 @default.
• W2773648825 hasConceptScore W2773648825C181199279 @default.
• W2773648825 hasConceptScore W2773648825C185592680 @default.
• W2773648825 hasConceptScore W2773648825C2780113650 @default.
• W2773648825 hasConceptScore W2773648825C2780841973 @default.
• W2773648825 hasConceptScore W2773648825C43617362 @default.
• W2773648825 hasConceptScore W2773648825C538909803 @default.
• W2773648825 hasConceptScore W2773648825C55493867 @default.
• W2773648825 hasFunder F4320321001 @default.
• W2773648825 hasLocation W27736488251 @default.
• W2773648825 hasLocation W27736488252 @default.
• W2773648825 hasOpenAccess W2773648825 @default.
• W2773648825 hasPrimaryLocation W27736488251 @default.
• W2773648825 hasRelatedWork W1830334520 @default.
• W2773648825 hasRelatedWork W1970475868 @default.
• W2773648825 hasRelatedWork W2300492196 @default.
• W2773648825 hasRelatedWork W2312406897 @default.
• W2773648825 hasRelatedWork W2352674505 @default.
• W2773648825 hasRelatedWork W2364492369 @default.
• W2773648825 hasRelatedWork W2370393484 @default.
• W2773648825 hasRelatedWork W2374805150 @default.
• W2773648825 hasRelatedWork W2390862174 @default.
• W2773648825 hasRelatedWork W2601524031 @default.
• W2773648825 hasVolume "215" @default.
• W2773648825 isParatext "false" @default.
• W2773648825 isRetracted "false" @default.
• W2773648825 magId "2773648825" @default.
• W2773648825 workType "article" @default.