Matches in SemOpenAlex for { <https://semopenalex.org/work/W2773825812> ?p ?o ?g. }
- W2773825812 endingPage "e1007127" @default.
- W2773825812 startingPage "e1007127" @default.
- W2773825812 abstract "In recent years, highly detailed characterization of adult bone marrow (BM) myeloid progenitors has been achieved and, as a result, the impact of somatic defects on different hematopoietic lineage fate decisions can be precisely determined. Fetal liver (FL) hematopoietic progenitor cells (HPCs) are poorly characterized in comparison, potentially hindering the study of the impact of genetic alterations on midgestation hematopoiesis. Numerous disorders, for example infant acute leukemias, have in utero origins and their study would therefore benefit from the ability to isolate highly purified progenitor subsets. We previously demonstrated that a Runx1 distal promoter (P1)-GFP::proximal promoter (P2)-hCD4 dual-reporter mouse (Mus musculus) model can be used to identify adult BM progenitor subsets with distinct lineage preferences. In this study, we undertook the characterization of the expression of Runx1-P1-GFP and P2-hCD4 in FL. Expression of P2-hCD4 in the FL immunophenotypic Megakaryocyte-Erythroid Progenitor (MEP) and Common Myeloid Progenitor (CMP) compartments corresponded to increased granulocytic/monocytic/megakaryocytic and decreased erythroid specification. Moreover, Runx1-P2-hCD4 expression correlated with several endogenous cell surface markers’ expression, including CD31 and CD45, providing a new strategy for prospective identification of highly purified fetal myeloid progenitors in transgenic mouse models. We utilized this methodology to compare the impact of the deletion of either total RUNX1 or RUNX1C alone and to determine the fetal HPCs lineages most substantially affected. This new prospective identification of FL progenitors therefore raises the prospect of identifying the underlying gene networks responsible with greater precision than previously possible." @default.
- W2773825812 created "2017-12-22" @default.
- W2773825812 creator A5013394786 @default.
- W2773825812 creator A5016021594 @default.
- W2773825812 creator A5022535195 @default.
- W2773825812 creator A5028642950 @default.
- W2773825812 creator A5030184193 @default.
- W2773825812 creator A5039517391 @default.
- W2773825812 creator A5065117902 @default.
- W2773825812 creator A5069020237 @default.
- W2773825812 date "2018-01-04" @default.
- W2773825812 modified "2023-09-26" @default.
- W2773825812 title "A novel prospective isolation of murine fetal liver progenitors to study in utero hematopoietic defects" @default.
- W2773825812 cites W1524329323 @default.
- W2773825812 cites W1739103212 @default.
- W2773825812 cites W1884163406 @default.
- W2773825812 cites W1932316892 @default.
- W2773825812 cites W1937332911 @default.
- W2773825812 cites W1952464171 @default.
- W2773825812 cites W1967892265 @default.
- W2773825812 cites W1970081233 @default.
- W2773825812 cites W1974520072 @default.
- W2773825812 cites W1974659776 @default.
- W2773825812 cites W1979058918 @default.
- W2773825812 cites W1982130774 @default.
- W2773825812 cites W1987798109 @default.
- W2773825812 cites W1990565971 @default.
- W2773825812 cites W1994254285 @default.
- W2773825812 cites W1997970567 @default.
- W2773825812 cites W2003459509 @default.
- W2773825812 cites W2004180374 @default.
- W2773825812 cites W2006231415 @default.
- W2773825812 cites W2010586398 @default.
- W2773825812 cites W2010934897 @default.
- W2773825812 cites W2011096612 @default.
- W2773825812 cites W2017208415 @default.
- W2773825812 cites W2017454328 @default.
- W2773825812 cites W2019552331 @default.
- W2773825812 cites W2020541351 @default.
- W2773825812 cites W2022474104 @default.
- W2773825812 cites W2026721712 @default.
- W2773825812 cites W2027610354 @default.
- W2773825812 cites W2030309930 @default.
- W2773825812 cites W2030566923 @default.
- W2773825812 cites W2033242304 @default.
- W2773825812 cites W2037628646 @default.
- W2773825812 cites W2038282968 @default.
- W2773825812 cites W2041172172 @default.
- W2773825812 cites W2051600987 @default.
- W2773825812 cites W2052532025 @default.
- W2773825812 cites W2052670712 @default.
- W2773825812 cites W2053220878 @default.
- W2773825812 cites W2054449135 @default.
- W2773825812 cites W2066721422 @default.
- W2773825812 cites W2069332674 @default.
- W2773825812 cites W2069659115 @default.
- W2773825812 cites W2070015044 @default.
- W2773825812 cites W2071991444 @default.
- W2773825812 cites W2073648680 @default.
- W2773825812 cites W2074267240 @default.
- W2773825812 cites W2077330278 @default.
- W2773825812 cites W2077383117 @default.
- W2773825812 cites W2082414007 @default.
- W2773825812 cites W2084407030 @default.
- W2773825812 cites W2086370314 @default.
- W2773825812 cites W2090786449 @default.
- W2773825812 cites W2093200886 @default.
- W2773825812 cites W2096477855 @default.
- W2773825812 cites W2098161879 @default.
- W2773825812 cites W2102015925 @default.
- W2773825812 cites W2102782482 @default.
- W2773825812 cites W2110918588 @default.
- W2773825812 cites W2110981938 @default.
- W2773825812 cites W2114876320 @default.
- W2773825812 cites W2115908301 @default.
- W2773825812 cites W2118258530 @default.
- W2773825812 cites W2122156001 @default.
- W2773825812 cites W2129141759 @default.
- W2773825812 cites W2129955536 @default.
- W2773825812 cites W2130727132 @default.
- W2773825812 cites W2130921299 @default.
- W2773825812 cites W2134526812 @default.
- W2773825812 cites W2141992541 @default.
- W2773825812 cites W2144470070 @default.
- W2773825812 cites W2152414109 @default.
- W2773825812 cites W2154699417 @default.
- W2773825812 cites W2157187112 @default.
- W2773825812 cites W2158060003 @default.
- W2773825812 cites W2160811005 @default.
- W2773825812 cites W2163803815 @default.
- W2773825812 cites W2168826120 @default.
- W2773825812 cites W2169456326 @default.
- W2773825812 cites W2171689896 @default.
- W2773825812 cites W2203817162 @default.
- W2773825812 cites W2266966492 @default.
- W2773825812 cites W2327451829 @default.
- W2773825812 cites W2340310005 @default.
- W2773825812 cites W2408400915 @default.