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• W2773907656 abstract "HomePlant DiseaseVol. 102, No. 2First Report of Brome mosaic virus Infecting Soybean, Isolated in Manitoba, Canada PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Brome mosaic virus Infecting Soybean, Isolated in Manitoba, CanadaG. A. Díaz-Cruz, C. M. Smith, K. F. Wiebe, J. M. Charette, and B. J. CassoneG. A. Díaz-CruzSearch for more papers by this author, C. M. SmithSearch for more papers by this author, K. F. WiebeSearch for more papers by this author, J. M. CharetteSearch for more papers by this author, and B. J. Cassone†Corresponding author: B. J. Cassone; E-mail: E-mail Address: cassoneb@brandonu.caSearch for more papers by this authorAffiliationsAuthors and Affiliations G. A. Díaz-Cruz C. M. Smith K. F. Wiebe , Department of Biology, Brandon University, Brandon, MB, R78 6A9, Canada J. M. Charette , Department of Chemistry, Brandon University, Brandon, MB, R78 6A9, Canada B. J. Cassone † , Department of Biology, Brandon University, Brandon, MB, R78 6A9, Canada. Published Online:6 Dec 2017https://doi.org/10.1094/PDIS-07-17-1012-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Brome mosaic virus (BMV) is a single-stranded positive-sense RNA virus belonging to the genus Bromovirus. BMV commonly infects bromegrass (Bromus inermis Leyss.) and other grass species. It is also one of the few grass viruses that infects dicotyledonous plants, including beet (Beta vulgaris) (Lane 1974), cucumber (Cucumis sativa) (Lane 1974), common bean (Phaseolus vulgaris) (Lane 1974), and cowpea (Vigna unguiculata) (Trzmiel et al. 2016). In August 2016, a soybean (Glycine max [L.] Merr.) leaf sample exhibiting dark spots with chlorotic halos distributed on different parts of the foliar tissue was collected from a soybean field in southern Manitoba, Canada. Total RNA was extracted from the leaf using the Plant DNA/RNA kit (Norgen Biotek) and sent to the Génome Québec Innovation Centre at McGill University for sequencing in 100-bp paired-end fashion on the Illumina HiSeq 2500 platform. Preprocessed reads were de novo assembled using CLC Genomics Workbench 10.0.1 software (CLC bio). Functional annotation of the resultant contigs was carried out using BLASTn on the NCBI nonredundant (nr) database (E value < 1 × 10−50). A total of 10 contigs (2,595 reads) of BMV origin were identified, ranging between 266 and 479 nt in length (GenBank accessions MF804328 to MF804337). All contigs had nucleotide identities >99% to BMV sequences deposited in the nr database, and all three 5′-capped RNAs were represented by more than one contig. To validate the RNA sequencing results, a two-step reverse transcription (RT) PCR was performed using custom-designed, BMV-specific primers (5′-ACATCACAATCTTGAGCTCGGT-3′ and 5′-CCGTTATGGTGGTGCCTTTG-3′). The PCR product was run on a 2% agarose gel, which yielded an amplicon of the expected 324 nt size. The PCR product was then purified using the Purelink Quick PCR Purification kit (Invitrogen) and sent for Sanger Sequencing at the Centre for Applied Genomics (Toronto, Canada) using both the forward and reverse PCR primers. The amplicon sequences obtained (323 nt) were compared using BLASTn against the NCBI nr database. They showed 99.7% nucleotide identity with BMV strain Fescue segment RNA1 (GenBank accession no. DQ530423), thus confirming BMV infection. To our knowledge, this is the first report of BMV infecting soybean. Although it is unlikely to cause significant economic impact to soybean, it is further evidence of BMV’s ability to infect both monocotyledon and dicotyledon plants.References:Lane, L. C. 1974. Adv. Virus Res. 19:151. Crossref, Google ScholarTrzmiel, K., et al. 2016. Eur. J. Plant Pathol. 145:233. https://doi.org/10.1007/s10658-015-0830-5 Crossref, ISI, Google ScholarThis work was supported by Manitoba Pulse & Soybean Growers (MPSG).DetailsFiguresLiterature CitedRelated Vol. 102, No. 2 February 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 19 Jan 2018Published: 6 Dec 2017Accepted: 7 Oct 2017 Page: 460 Information© 2018 The American Phytopathological SocietyCited byBrome mosaic virus detected in Kansas wheat co-infected with other common wheat viruses3 March 2023 | Frontiers in Plant Science, Vol. 14Weeds as Reservoirs of Viruses in Agrobiocenoses of Cereal Crops in Ukraine28 February 2023 | Mikrobiolohichnyi Zhurnal, Vol. 84, No. 6Brome mosaic virusCABI Compendium, Vol. CABI CompendiumCereal viruses – brome mosaic virus: prevalence and diagnosticsIOP Conference Series: Earth and Environmental Science, Vol. 663, No. 1Applications of Next-Generation Sequencing for Large-Scale Pathogen Diagnoses in SoybeanGustavo A. Díaz-Cruz, Charlotte M. Smith, Kiana F. Wiebe, Sachi M. Villanueva, Adam R. Klonowski, and Bryan J. Cassone22 April 2019 | Plant Disease, Vol. 103, No. 6Glycine max (Soybean)6 June 2020" @default.
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