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• W2773990804 abstract "Abstract Apical blebbing, a non‐classical secretion mechanism, occurs in the mature porcine epididymis as part of its normal function. Proteins secreted by this mechanism contribute to the modification of the sperm plasma membrane during epididymal transit and are thought to contribute to acquisition of fertilizing ability. However, little is known about the regulation of this secretion mechanism in an in vivo model. Previous work demonstrated apical blebbing in the epididymis developed pubertally, suggesting androgens, sperm or other luminal factors regulated this process. Hence, the objective was to evaluate the hypothesized regulation of apical blebbing in the epididymis of pubertal boars by androgens and luminal factors. Androgen receptor blockade (flutamide) and surgical interventions (efferent duct ligation, orchidectomy or transection of the caput epididymis) were used to alter signaling, and the subsequent effects on apical blebbing were evaluated histologically. Apical blebbing was not altered by androgen receptor blockade with flutamide, but was significantly reduced 24 h after efferent duct ligation and after orchidectomy, treatments that eliminated luminal flow from the testis ( P < 0.05). Like efferent duct ligation, epididymal transection altered luminal flow without removing the androgen source and significantly reduced the appearance of apical blebbing ( P < 0.05). In conclusion, apical blebbing in the porcine epididymis appears to be regulated by luminal factors." @default.
• W2773990804 created "2017-12-22" @default.
• W2773990804 creator A5017199339 @default.
• W2773990804 creator A5086435358 @default.
• W2773990804 date "2017-12-04" @default.
• W2773990804 modified "2023-10-14" @default.
• W2773990804 title "Regulation of apical blebbing in the porcine epididymis" @default.
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• W2773990804 doi "https://doi.org/10.1111/joa.12755" @default.
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