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• W2774037242 abstract "Abstract The purpose of this work was to scale-up the culture of grapevine cells (Vitis labrusca) from shake-flasks (100 mL) to a 5L stirred bioreactor in order to develop a model able to describe the bioproduction of resveratrol under the controlled conditions of a fed-batch culture. For this study, the biomass, resveratrol and sugar concentrations as well as pH and dissolved oxygen were monitored daily. The experiments were conducted twice over a three month period. The culture was elicited during the exponential growth phase with methyl jasmonate, leading the cells to exhibit a complex behaviour during the resveratrol production phase. A model of the system behaviour involving simple mechanisms is proposed and successfully confronted to the experimental results. This model demonstrates that the system dynamic can be decomposed into four phases: a lag phase (cell growth slowing down), a starting phase (beginning of resveratrol production), a surge phase (significant resveratrol production accompanied by significant cell death) and a stationary phase. Thus, we were able to successfully scale-up resveratrol production from 100 mL flasks to 5L bioreactors." @default.
• W2774037242 created "2017-12-22" @default.
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• W2774037242 date "2018-03-01" @default.
• W2774037242 modified "2023-09-23" @default.
• W2774037242 title "Resveratrol production by grapevine cells in fed-batch bioreactor: Experiments and modelling" @default.
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• W2774037242 doi "https://doi.org/10.1016/j.bej.2017.12.009" @default.
• W2774037242 hasPublicationYear "2018" @default.
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