FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2774122405> ?p ?o ?g. }

• W2774122405 endingPage "980" @default.
• W2774122405 startingPage "965" @default.
• W2774122405 abstract "Abstract Two‐dimensional gel electrophoresis (2DE) in proteomics is traditionally assumed to contain only one or two proteins in each 2DE spot. However, 2DE resolution is being complemented by the rapid development of high sensitivity mass spectrometers. Here we compared MALDI‐MS, LC‐Q‐TOF MS and LC‐Orbitrap Velos MS for the identification of proteins within one spot. With LC‐Orbitrap Velos MS each Coomassie Blue‐stained 2DE spot contained an average of at least 42 and 63 proteins/spot in an analysis of a human glioblastoma proteome and a human pituitary adenoma proteome, respectively, if a single gel spot was analyzed. If a pool of three matched gel spots was analyzed this number further increased up to an average of 230 and 118 proteins/spot for glioblastoma and pituitary adenoma proteome, respectively. Multiple proteins per spot confirm the necessity of isotopic labeling in large‐scale quantification of different protein species in a proteome. Furthermore, a protein abundance analysis revealed that most of the identified proteins in each analyzed 2DE spot were low‐abundance proteins. Many proteins were present in several of the analyzed spots showing the ability of 2DE‐MS to separate at the protein species level. Therefore, 2DE coupled with high‐sensitivity LC‐MS has a clearly higher sensitivity as expected until now to detect, identify and quantify low abundance proteins in a complex human proteome with an estimated resolution of about 500 000 protein species. This clearly exceeds the resolution power of bottom‐up LC‐MS investigations." @default.
• W2774122405 created "2017-12-22" @default.
• W2774122405 creator A5000238995 @default.
• W2774122405 creator A5001906823 @default.
• W2774122405 creator A5014615941 @default.
• W2774122405 creator A5021869615 @default.
• W2774122405 creator A5026259727 @default.
• W2774122405 creator A5031315906 @default.
• W2774122405 creator A5032654347 @default.
• W2774122405 creator A5034134778 @default.
• W2774122405 creator A5040689829 @default.
• W2774122405 creator A5048151775 @default.
• W2774122405 creator A5054875063 @default.
• W2774122405 creator A5055828140 @default.
• W2774122405 creator A5058524692 @default.
• W2774122405 creator A5059193881 @default.
• W2774122405 date "2018-02-08" @default.
• W2774122405 modified "2023-09-30" @default.
• W2774122405 title "How many proteins can be identified in a 2DE gel spot within an analysis of a complex human cancer tissue proteome?" @default.
• W2774122405 cites W1606423922 @default.
• W2774122405 cites W1797925867 @default.
• W2774122405 cites W1911608194 @default.
• W2774122405 cites W1939815238 @default.
• W2774122405 cites W1945748241 @default.
• W2774122405 cites W1966595445 @default.
• W2774122405 cites W1969297489 @default.
• W2774122405 cites W1970133737 @default.
• W2774122405 cites W1970824545 @default.
• W2774122405 cites W1971944746 @default.
• W2774122405 cites W1976752649 @default.
• W2774122405 cites W1980804108 @default.
• W2774122405 cites W1982930121 @default.
• W2774122405 cites W1990089990 @default.
• W2774122405 cites W2001200460 @default.
• W2774122405 cites W2025903813 @default.
• W2774122405 cites W2028156442 @default.
• W2774122405 cites W2033159891 @default.
• W2774122405 cites W2035382483 @default.
• W2774122405 cites W2036641811 @default.
• W2774122405 cites W2043103546 @default.
• W2774122405 cites W2045691764 @default.
• W2774122405 cites W2056499061 @default.
• W2774122405 cites W2058514352 @default.
• W2774122405 cites W2073391230 @default.
• W2774122405 cites W2080205650 @default.
• W2774122405 cites W2102796372 @default.
• W2774122405 cites W2104814618 @default.
• W2774122405 cites W2109908654 @default.
• W2774122405 cites W2111208057 @default.
• W2774122405 cites W2113376672 @default.
• W2774122405 cites W2117506397 @default.
• W2774122405 cites W2121234677 @default.
• W2774122405 cites W2124175382 @default.
• W2774122405 cites W2132851761 @default.
• W2774122405 cites W2135581618 @default.
• W2774122405 cites W2148490163 @default.
• W2774122405 cites W2157304217 @default.
• W2774122405 cites W2159358127 @default.
• W2774122405 cites W2164503710 @default.
• W2774122405 cites W2165396818 @default.
• W2774122405 cites W2165962849 @default.
• W2774122405 cites W2168909179 @default.
• W2774122405 cites W2233834663 @default.
• W2774122405 cites W2287622873 @default.
• W2774122405 cites W2518249674 @default.
• W2774122405 cites W3100235074 @default.
• W2774122405 cites W4296926797 @default.
• W2774122405 doi "https://doi.org/10.1002/elps.201700330" @default.
• W2774122405 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29205401" @default.
• W2774122405 hasPublicationYear "2018" @default.
• W2774122405 type Work @default.
• W2774122405 sameAs 2774122405 @default.
• W2774122405 citedByCount "51" @default.
• W2774122405 countsByYear W27741224052018 @default.
• W2774122405 countsByYear W27741224052019 @default.
• W2774122405 countsByYear W27741224052020 @default.
• W2774122405 countsByYear W27741224052021 @default.
• W2774122405 countsByYear W27741224052022 @default.
• W2774122405 countsByYear W27741224052023 @default.
• W2774122405 crossrefType "journal-article" @default.
• W2774122405 hasAuthorship W2774122405A5000238995 @default.
• W2774122405 hasAuthorship W2774122405A5001906823 @default.
• W2774122405 hasAuthorship W2774122405A5014615941 @default.
• W2774122405 hasAuthorship W2774122405A5021869615 @default.
• W2774122405 hasAuthorship W2774122405A5026259727 @default.
• W2774122405 hasAuthorship W2774122405A5031315906 @default.
• W2774122405 hasAuthorship W2774122405A5032654347 @default.
• W2774122405 hasAuthorship W2774122405A5034134778 @default.
• W2774122405 hasAuthorship W2774122405A5040689829 @default.
• W2774122405 hasAuthorship W2774122405A5048151775 @default.
• W2774122405 hasAuthorship W2774122405A5054875063 @default.
• W2774122405 hasAuthorship W2774122405A5055828140 @default.
• W2774122405 hasAuthorship W2774122405A5058524692 @default.
• W2774122405 hasAuthorship W2774122405A5059193881 @default.
• W2774122405 hasBestOaLocation W27741224052 @default.
• W2774122405 hasConcept C104317684 @default.
• W2774122405 hasConcept C104397665 @default.
• W2774122405 hasConcept C116084860 @default.

• next