FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2774320934> ?p ?o ?g. }

• W2774320934 endingPage "131" @default.
• W2774320934 startingPage "124" @default.
• W2774320934 abstract "Background: In this study we investigated the function of the non-catalytic region of tyrosine kinase adaptor protein 2 (NCK2) and its correlation with ITGB1 and ITGB4 integrins in driving ovarian cancer (OvCa) aggressiveness. We also evaluated whether NCK2 may influence prognosis in OvCa patients. Methods: Nanofluidic technology was used to analyze expression of NCK2 in 332 OvCa patients. To evaluate mRNA expression of NCK2, integrins and VEGFA in OvCa cell lines, qRT-PCR was performed. Stable NCK2 overexpression was obtained in OVCAR3. qRT-PCR and Western blot were performed to evaluate expression changes of VEGFA, vimentin, ITGB1, ITGB4, MMP2 and MMP9 under normoxia and hypoxia conditions. Coimmunoprecipitation (Co-IP) was performed in the A2780 cell line to study the interaction between NCK2 and proteins of interest. To investigate whether NCK2 can influence anchorage-independent growth, a soft agar assay was completed. Transwell invasion assay was performed on stable-transfected OVCAR-3 cell lines. Results: Nanofluidic data showed NCK2 can play an important role as a factor promoting tumor aggressiveness and survival in OvCa. This role was also linked to the behaviors of ITGB1 and ITGB4. Moreover, in cells overexpressing NCK2, the expression of vimentin, MMP2, MMP9, VEGFA and ITGB1, but not of ITGB4 was induced by hypoxia. Co-IP showed that NCK2 can directly bind ITGB1, but not VEGFA. NCK2 may be involved in mediating cell-extracellular matrix interactions in OvCa cells by influencing tumor aggressiveness. Conclusions: This study provides evidence of a possible role of NCK2 as biomarker of OvCa progression." @default.
• W2774320934 created "2017-12-22" @default.
• W2774320934 creator A5006321312 @default.
• W2774320934 creator A5019093366 @default.
• W2774320934 creator A5020790343 @default.
• W2774320934 creator A5024731355 @default.
• W2774320934 creator A5026048739 @default.
• W2774320934 creator A5045785533 @default.
• W2774320934 creator A5047616963 @default.
• W2774320934 creator A5056285210 @default.
• W2774320934 creator A5073293203 @default.
• W2774320934 date "2017-05-12" @default.
• W2774320934 modified "2023-10-17" @default.
• W2774320934 title "Non-catalytic region of tyrosine kinase adaptor protein 2 (NCK2) pathways as factor promoting aggressiveness in ovarian cancer" @default.
• W2774320934 cites W1577577364 @default.
• W2774320934 cites W1964211098 @default.
• W2774320934 cites W1977509902 @default.
• W2774320934 cites W1984690727 @default.
• W2774320934 cites W1984829593 @default.
• W2774320934 cites W1997338647 @default.
• W2774320934 cites W2001282311 @default.
• W2774320934 cites W2007984671 @default.
• W2774320934 cites W2015522187 @default.
• W2774320934 cites W2017951227 @default.
• W2774320934 cites W2020904443 @default.
• W2774320934 cites W2025531664 @default.
• W2774320934 cites W2029231027 @default.
• W2774320934 cites W2037761545 @default.
• W2774320934 cites W2065530077 @default.
• W2774320934 cites W2066105836 @default.
• W2774320934 cites W2066616821 @default.
• W2774320934 cites W2073237741 @default.
• W2774320934 cites W2084126321 @default.
• W2774320934 cites W2084243350 @default.
• W2774320934 cites W2087145616 @default.
• W2774320934 cites W2087963453 @default.
• W2774320934 cites W2099380130 @default.
• W2774320934 cites W2123713581 @default.
• W2774320934 cites W2126496132 @default.
• W2774320934 cites W2129290128 @default.
• W2774320934 cites W2130839996 @default.
• W2774320934 cites W2147000598 @default.
• W2774320934 cites W2153575042 @default.
• W2774320934 cites W2162371443 @default.
• W2774320934 cites W2175618680 @default.
• W2774320934 doi "https://doi.org/10.5301/ijbm.5000264" @default.
• W2774320934 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29218693" @default.
• W2774320934 hasPublicationYear "2017" @default.
• W2774320934 type Work @default.
• W2774320934 sameAs 2774320934 @default.
• W2774320934 citedByCount "7" @default.
• W2774320934 countsByYear W27743209342020 @default.
• W2774320934 countsByYear W27743209342021 @default.
• W2774320934 countsByYear W27743209342022 @default.
• W2774320934 countsByYear W27743209342023 @default.
• W2774320934 crossrefType "journal-article" @default.
• W2774320934 hasAuthorship W2774320934A5006321312 @default.
• W2774320934 hasAuthorship W2774320934A5019093366 @default.
• W2774320934 hasAuthorship W2774320934A5020790343 @default.
• W2774320934 hasAuthorship W2774320934A5024731355 @default.
• W2774320934 hasAuthorship W2774320934A5026048739 @default.
• W2774320934 hasAuthorship W2774320934A5045785533 @default.
• W2774320934 hasAuthorship W2774320934A5047616963 @default.
• W2774320934 hasAuthorship W2774320934A5056285210 @default.
• W2774320934 hasAuthorship W2774320934A5073293203 @default.
• W2774320934 hasBestOaLocation W27743209341 @default.
• W2774320934 hasConcept C104317684 @default.
• W2774320934 hasConcept C121608353 @default.
• W2774320934 hasConcept C127561419 @default.
• W2774320934 hasConcept C146285616 @default.
• W2774320934 hasConcept C147447768 @default.
• W2774320934 hasConcept C167734588 @default.
• W2774320934 hasConcept C203014093 @default.
• W2774320934 hasConcept C204232928 @default.
• W2774320934 hasConcept C2775997469 @default.
• W2774320934 hasConcept C2777025900 @default.
• W2774320934 hasConcept C2779013556 @default.
• W2774320934 hasConcept C2780394083 @default.
• W2774320934 hasConcept C2780427987 @default.
• W2774320934 hasConcept C2780699576 @default.
• W2774320934 hasConcept C502942594 @default.
• W2774320934 hasConcept C54355233 @default.
• W2774320934 hasConcept C55493867 @default.
• W2774320934 hasConcept C86803240 @default.
• W2774320934 hasConceptScore W2774320934C104317684 @default.
• W2774320934 hasConceptScore W2774320934C121608353 @default.
• W2774320934 hasConceptScore W2774320934C127561419 @default.
• W2774320934 hasConceptScore W2774320934C146285616 @default.
• W2774320934 hasConceptScore W2774320934C147447768 @default.
• W2774320934 hasConceptScore W2774320934C167734588 @default.
• W2774320934 hasConceptScore W2774320934C203014093 @default.
• W2774320934 hasConceptScore W2774320934C204232928 @default.
• W2774320934 hasConceptScore W2774320934C2775997469 @default.
• W2774320934 hasConceptScore W2774320934C2777025900 @default.
• W2774320934 hasConceptScore W2774320934C2779013556 @default.
• W2774320934 hasConceptScore W2774320934C2780394083 @default.
• W2774320934 hasConceptScore W2774320934C2780427987 @default.
• W2774320934 hasConceptScore W2774320934C2780699576 @default.

• next