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- W2774399578 abstract "AbstractObjective of this study was to evaluate the effect of supplementation of growing rabbits’ diet with 5% spirulina (Arthrospira platensis) and/or 3% (Thymus vulgaris L.), on composition and amount of rabbits caecal microbiota. After weaning, rabbits were randomly sorted to 4 groups (n = 42/group). The control group received a pellet without any supplementation, in treated groups the dietary supplementation was provided until 77 days of age. On 49, 63 and 77 days of age, six healthy animals/group were randomly selected and slaughtered. From the caecal content the amount of total bacteria, Bacteroides, Clostridium leptum and Clostridium coccoides were determined by quantitative polymerase chain reaction (qPCR), with the aid of bacterial ribosome coding DNA. The copy number of total bacteria, C. leptum, C. coccoides and Bacteroides varied between 2.75 × 1012 to 2.24 × 1013, 5.25 × 1011 to 1.82 × 1012, 2.5 × 1010 to 6.91 × 1011 and 5.89 × 1010 to 1.10 × 1012, respectively. The amount of investigated bac..." @default.
- W2774399578 created "2017-12-22" @default.
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- W2774399578 date "2018-07-01" @default.
- W2774399578 modified "2023-10-17" @default.
- W2774399578 title "Quantitative PCR with 16S rRNA-gene-targeted specific primers for analysis of caecal microbial community in growing rabbits after dietary supplementation of thyme (Thymus vulgaris) and spirulina (Arthrospira platensis)" @default.
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- W2774399578 doi "https://doi.org/10.1080/1828051x.2017.1400413" @default.
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