Matches in SemOpenAlex for { <https://semopenalex.org/work/W2774839461> ?p ?o ?g. }
- W2774839461 endingPage "1374" @default.
- W2774839461 startingPage "1365" @default.
- W2774839461 abstract "The metabolites of steroid hormones catalyzed by cytochrome P450s (CYPs) play a significant role in carcinogenicity. Herein we report a facile electrochemical liver microsomal bioreactor based on the polydopamine encapsulated gold-graphene nanocomposite (PDA/[email protected]) for the fast steroid hormone metabolism assays. In this work, human liver microsomes (HLMs) were directly attached on PDA/[email protected] without any activation process. Besides, due to the high conductivity, large active areas and good biocompatibility of PDA/[email protected], the direct electron transfer of HLM with the rate constant (ks) of 8.9 s−1 was achieved and the bioactivity of membrane bound proteins in HLM was largely maintained. Compared with recombinant enzymes, the immobilized HLM displayed obviously lower apparent Michaelis-Menten constant (Kmapp) of 175 ± 20 μM. Through HPLC-MS and CYP-specific inhibition experiment, the electrode-driven catalytic behavior of HLM for the conversion of testosterone to 6βOHtestosterone was confirmed. Moreover, the constructed HLM bioreactor was also successfully applied to the metabolism of other two steroid hormones (estrone and progesterone). This work has immense significance in the design of practically viable HLM bioreactors for the toxicity analysis of steroid hormones and stereoselective metabolite synthesis." @default.
- W2774839461 created "2017-12-22" @default.
- W2774839461 creator A5020482805 @default.
- W2774839461 creator A5022876276 @default.
- W2774839461 creator A5029081960 @default.
- W2774839461 creator A5045363491 @default.
- W2774839461 creator A5049108933 @default.
- W2774839461 creator A5079315299 @default.
- W2774839461 creator A5083926193 @default.
- W2774839461 date "2017-12-01" @default.
- W2774839461 modified "2023-09-29" @default.
- W2774839461 title "Fast steroid hormone metabolism assays with electrochemical liver microsomal bioreactor based on polydopamine encapsulated gold-graphene nanocomposite" @default.
- W2774839461 cites W1872245393 @default.
- W2774839461 cites W1884330844 @default.
- W2774839461 cites W1921989175 @default.
- W2774839461 cites W1963499355 @default.
- W2774839461 cites W1963651611 @default.
- W2774839461 cites W1977946626 @default.
- W2774839461 cites W1985348198 @default.
- W2774839461 cites W1990630304 @default.
- W2774839461 cites W1993664411 @default.
- W2774839461 cites W2004372265 @default.
- W2774839461 cites W2006636051 @default.
- W2774839461 cites W2006891910 @default.
- W2774839461 cites W2009463903 @default.
- W2774839461 cites W2015139372 @default.
- W2774839461 cites W2015753811 @default.
- W2774839461 cites W2029906456 @default.
- W2774839461 cites W2041685866 @default.
- W2774839461 cites W2044494844 @default.
- W2774839461 cites W2046649550 @default.
- W2774839461 cites W2060067888 @default.
- W2774839461 cites W2063342318 @default.
- W2774839461 cites W2067859522 @default.
- W2774839461 cites W2069527511 @default.
- W2774839461 cites W2075390160 @default.
- W2774839461 cites W2075812888 @default.
- W2774839461 cites W2079814086 @default.
- W2774839461 cites W2081495308 @default.
- W2774839461 cites W2082262322 @default.
- W2774839461 cites W2092858957 @default.
- W2774839461 cites W2114006399 @default.
- W2774839461 cites W2133997092 @default.
- W2774839461 cites W2141239229 @default.
- W2774839461 cites W2217025161 @default.
- W2774839461 cites W2291684404 @default.
- W2774839461 cites W2313815402 @default.
- W2774839461 cites W2319342274 @default.
- W2774839461 cites W2321282239 @default.
- W2774839461 cites W2321685432 @default.
- W2774839461 cites W2325137743 @default.
- W2774839461 cites W2333817238 @default.
- W2774839461 cites W2519958047 @default.
- W2774839461 cites W4233194460 @default.
- W2774839461 cites W4255068297 @default.
- W2774839461 cites W569938954 @default.
- W2774839461 cites W950131151 @default.
- W2774839461 doi "https://doi.org/10.1016/j.electacta.2017.11.195" @default.
- W2774839461 hasPublicationYear "2017" @default.
- W2774839461 type Work @default.
- W2774839461 sameAs 2774839461 @default.
- W2774839461 citedByCount "12" @default.
- W2774839461 countsByYear W27748394612018 @default.
- W2774839461 countsByYear W27748394612019 @default.
- W2774839461 countsByYear W27748394612020 @default.
- W2774839461 countsByYear W27748394612021 @default.
- W2774839461 countsByYear W27748394612022 @default.
- W2774839461 countsByYear W27748394612023 @default.
- W2774839461 crossrefType "journal-article" @default.
- W2774839461 hasAuthorship W2774839461A5020482805 @default.
- W2774839461 hasAuthorship W2774839461A5022876276 @default.
- W2774839461 hasAuthorship W2774839461A5029081960 @default.
- W2774839461 hasAuthorship W2774839461A5045363491 @default.
- W2774839461 hasAuthorship W2774839461A5049108933 @default.
- W2774839461 hasAuthorship W2774839461A5079315299 @default.
- W2774839461 hasAuthorship W2774839461A5083926193 @default.
- W2774839461 hasConcept C168170006 @default.
- W2774839461 hasConcept C178790620 @default.
- W2774839461 hasConcept C181199279 @default.
- W2774839461 hasConcept C185592680 @default.
- W2774839461 hasConcept C2777477808 @default.
- W2774839461 hasConcept C2780902042 @default.
- W2774839461 hasConcept C526171541 @default.
- W2774839461 hasConcept C55493867 @default.
- W2774839461 hasConcept C62231903 @default.
- W2774839461 hasConcept C71315377 @default.
- W2774839461 hasConcept C87644729 @default.
- W2774839461 hasConceptScore W2774839461C168170006 @default.
- W2774839461 hasConceptScore W2774839461C178790620 @default.
- W2774839461 hasConceptScore W2774839461C181199279 @default.
- W2774839461 hasConceptScore W2774839461C185592680 @default.
- W2774839461 hasConceptScore W2774839461C2777477808 @default.
- W2774839461 hasConceptScore W2774839461C2780902042 @default.
- W2774839461 hasConceptScore W2774839461C526171541 @default.
- W2774839461 hasConceptScore W2774839461C55493867 @default.
- W2774839461 hasConceptScore W2774839461C62231903 @default.
- W2774839461 hasConceptScore W2774839461C71315377 @default.
- W2774839461 hasConceptScore W2774839461C87644729 @default.