Matches in SemOpenAlex for { <https://semopenalex.org/work/W2775373670> ?p ?o ?g. }
- W2775373670 abstract "New Delhi metallo-β-lactamases (NDMs), the recent additions to metallo-β-lactamases (MBLs), pose a serious public health threat due to its highly efficient hydrolysis of β-lactam antibiotics and rapid worldwide dissemination. The MBL-hydrolyzing mechanism for carbapenems is less studied than that of penicillins and cephalosporins. Here, we report crystal structures of NDM-1 in complex with hydrolyzed imipenem and meropenem, at resolutions of 1.80-2.32 Å, together with NMR spectra monitoring meropenem hydrolysis. Three enzyme-intermediate/product derivatives, EI1, EI2, and EP, are trapped in these crystals. Our structural data reveal double-bond tautomerization from Δ2 to Δ1, absence of a bridging water molecule and an exclusive β-diastereomeric product, all suggesting that the hydrolytic intermediates are protonated by a bulky water molecule incoming from the β-face. These results strongly suggest a distinct mechanism of NDM-1-catalyzed carbapenem hydrolysis from that of penicillin or cephalosporin hydrolysis, which may provide a novel rationale for design of mechanism-based inhibitors." @default.
- W2775373670 created "2017-12-22" @default.
- W2775373670 creator A5015948426 @default.
- W2775373670 creator A5033111996 @default.
- W2775373670 creator A5044330539 @default.
- W2775373670 creator A5060414497 @default.
- W2775373670 creator A5071037763 @default.
- W2775373670 creator A5080749555 @default.
- W2775373670 date "2017-12-01" @default.
- W2775373670 modified "2023-10-15" @default.
- W2775373670 title "The mechanism of NDM-1-catalyzed carbapenem hydrolysis is distinct from that of penicillin or cephalosporin hydrolysis" @default.
- W2775373670 cites W1594633721 @default.
- W2775373670 cites W1619683848 @default.
- W2775373670 cites W1895007034 @default.
- W2775373670 cites W1971186057 @default.
- W2775373670 cites W1978212838 @default.
- W2775373670 cites W1978484656 @default.
- W2775373670 cites W1982891627 @default.
- W2775373670 cites W1993400812 @default.
- W2775373670 cites W1994530471 @default.
- W2775373670 cites W2015696076 @default.
- W2775373670 cites W2016230165 @default.
- W2775373670 cites W2018300096 @default.
- W2775373670 cites W2022133651 @default.
- W2775373670 cites W2026219411 @default.
- W2775373670 cites W2034903735 @default.
- W2775373670 cites W2036171075 @default.
- W2775373670 cites W2040651669 @default.
- W2775373670 cites W2046681103 @default.
- W2775373670 cites W2055140709 @default.
- W2775373670 cites W2058232170 @default.
- W2775373670 cites W2059355492 @default.
- W2775373670 cites W2079115916 @default.
- W2775373670 cites W2086441450 @default.
- W2775373670 cites W2087126312 @default.
- W2775373670 cites W2088037578 @default.
- W2775373670 cites W2094018144 @default.
- W2775373670 cites W2112625634 @default.
- W2775373670 cites W2113518689 @default.
- W2775373670 cites W2114367386 @default.
- W2775373670 cites W2123677996 @default.
- W2775373670 cites W2124026197 @default.
- W2775373670 cites W2128950873 @default.
- W2775373670 cites W2129454182 @default.
- W2775373670 cites W2130239585 @default.
- W2775373670 cites W2136653513 @default.
- W2775373670 cites W2143874510 @default.
- W2775373670 cites W2146956670 @default.
- W2775373670 cites W2154197653 @default.
- W2775373670 cites W2154714625 @default.
- W2775373670 cites W2158147502 @default.
- W2775373670 cites W2162014979 @default.
- W2775373670 cites W2270393624 @default.
- W2775373670 cites W2323783024 @default.
- W2775373670 cites W2419326809 @default.
- W2775373670 cites W24471328 @default.
- W2775373670 cites W2607580877 @default.
- W2775373670 cites W2785436589 @default.
- W2775373670 cites W4248872320 @default.
- W2775373670 doi "https://doi.org/10.1038/s41467-017-02339-w" @default.
- W2775373670 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5740130" @default.
- W2775373670 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29269938" @default.
- W2775373670 hasPublicationYear "2017" @default.
- W2775373670 type Work @default.
- W2775373670 sameAs 2775373670 @default.
- W2775373670 citedByCount "79" @default.
- W2775373670 countsByYear W27753736702018 @default.
- W2775373670 countsByYear W27753736702019 @default.
- W2775373670 countsByYear W27753736702020 @default.
- W2775373670 countsByYear W27753736702021 @default.
- W2775373670 countsByYear W27753736702022 @default.
- W2775373670 countsByYear W27753736702023 @default.
- W2775373670 crossrefType "journal-article" @default.
- W2775373670 hasAuthorship W2775373670A5015948426 @default.
- W2775373670 hasAuthorship W2775373670A5033111996 @default.
- W2775373670 hasAuthorship W2775373670A5044330539 @default.
- W2775373670 hasAuthorship W2775373670A5060414497 @default.
- W2775373670 hasAuthorship W2775373670A5071037763 @default.
- W2775373670 hasAuthorship W2775373670A5080749555 @default.
- W2775373670 hasBestOaLocation W27753736701 @default.
- W2775373670 hasConcept C111233374 @default.
- W2775373670 hasConcept C178790620 @default.
- W2775373670 hasConcept C181199279 @default.
- W2775373670 hasConcept C185592680 @default.
- W2775373670 hasConcept C21951064 @default.
- W2775373670 hasConcept C2776685102 @default.
- W2775373670 hasConcept C2778368673 @default.
- W2775373670 hasConcept C2779375183 @default.
- W2775373670 hasConcept C2779631663 @default.
- W2775373670 hasConcept C2780120296 @default.
- W2775373670 hasConcept C501593827 @default.
- W2775373670 hasConcept C55493867 @default.
- W2775373670 hasConcept C64778159 @default.
- W2775373670 hasConcept C71240020 @default.
- W2775373670 hasConcept C94412978 @default.
- W2775373670 hasConcept C94665300 @default.
- W2775373670 hasConceptScore W2775373670C111233374 @default.
- W2775373670 hasConceptScore W2775373670C178790620 @default.
- W2775373670 hasConceptScore W2775373670C181199279 @default.
- W2775373670 hasConceptScore W2775373670C185592680 @default.