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- W2775408089 abstract "The extensive use of polybrominated diphenyl ethers (PBDEs) has resulted in its increasingly widespread presence. Especially the lower halogenated PBDEs accumulate to a greater degree than the higher halogenated PBDEs in house dust, sewage sludge, pets, or even humans. In the present work, we developed an ultrasensitive biotin-streptavidin-enhanced carbon nanotube amplification strategy for the immunodetection of PBDEs, in which single-walled carbon nanotubes were used to immobilize numerous streptavidin. Meanwhile, we used biotin conjugated horseradish peroxidase (B-HRP) and biotin conjugated Goat anti-rabbit (B-IgG) to link the HRP and IgG to CNTs by using a biotin-streptavidin system. The sensitivity of the streptavidin-biotin-IgG-CNTs-HRP bioconjugate was compared with a commercial HRP-labelled IgG by using indirect competitive ELISA. The limit of this proposed ELISA detection (IC10) was 0.0059 ng/mL, showing a 20-time lower detection limit over the commercial one (IC10 = 0.1193 ng/mL). Finally, we applied the assay to the detection of PBDEs in dust samples. The results were consistent with those using GC-ECD, which confirmed that the proposed amplification strategy was accurate and receptive. This proposed biotin-streptavidin-enhanced carbon nanotube amplification strategy would be useful for ultrasensitive immunodetection in environmental studies." @default.
- W2775408089 created "2017-12-22" @default.
- W2775408089 creator A5070775744 @default.
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- W2775408089 date "2017-12-01" @default.
- W2775408089 modified "2023-10-03" @default.
- W2775408089 title "A Biotin-streptavidin-enhanced Carbon Nanotube Amplification Strategy for an Ultrasensitive Immunodetection of Polybrominated Diphenyl Ethers" @default.
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- W2775408089 doi "https://doi.org/10.2116/analsci.33.1441" @default.
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