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• W2775530028 startingPage "11046" @default.
• W2775530028 abstract "Cellulase and mannanase are both important enzyme additives in animal feeds. Expressing the two enzymes simultaneously within one microbial host could potentially lead to cost reductions in the feeding of animals. For this purpose, we codon-optimized the Aspergillus niger Man5A gene to the codon-usage bias of Trichoderma reesei. By comparing the free energies and the local structures of the nucleotide sequences, one optimized sequence was finally selected and transformed into the T. reesei pyridine-auxotrophic strain TU-6. The codon-optimized gene was expressed to a higher level than the original one. Further expressing the codon-optimized gene in a mutated T. reesei strain through fed-batch cultivation resulted in coproduction of cellulase and mannanase up to 1376 U·mL–1 and 1204 U·mL–1, respectively." @default.
• W2775530028 created "2017-12-22" @default.
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• W2775530028 date "2017-12-08" @default.
• W2775530028 modified "2023-09-23" @default.
• W2775530028 title "Efficient Coproduction of Mannanase and Cellulase by the Transformation of a Codon-Optimized Endomannanase Gene from <i>Aspergillus niger</i> into <i>Trichoderma reesei</i>" @default.
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• W2775530028 doi "https://doi.org/10.1021/acs.jafc.7b05114" @default.
• W2775530028 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29199828" @default.
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