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• W2775604456 abstract "The new Sysmex UF‐1000i analyzer – which incorporates bacteria morphology distinction – allows to automatically screen samples to be cultured at microbiology laboratories. We have evaluated the feasibility and accuracy of Sysmex UF‐1000i to screen urinary tract infections (UTIs). A total amount of 2468 urine samples from six Spanish hospitals were analysed. Demographic and clinical data such as age, gender, source and sample type, preserving conditions, cytometer parameters (bacteria, leucocytes and bacteria morphology) as well as urine culture results (gold standard) were recorded. After applying data mining techniques, the variables of age, bacteria count and rod morphology were defined as predictive variables of UTIs. By using the UF‐1000i in combination with a predictive algorithm of three decision rules, we could identify 94·9 and 47·4% positive and negative urine samples, respectively, with a negative predictive value of 97 and only 1·17% diagnostic error. This error was reduced down to 0·4% when contaminated samples were excluded. Our results show that flow cytometry parameters together with age, by means of a predictive algorithm model, can be used to screen UTIs. Its implementation would avoid culturing 38% of urine samples, and therefore, would reduce time to diagnosis with a discrete false negative ratio. Fluorescent flow cytometry performance has recently spread for urine screening. However, controversy about cytometer results can be drawn from medical literature. This study shows the diagnosis accuracy of Sysmex UF‐1000i analyzer by means of a group of decision rules encompassing both demographic variables (age) and cytometer parameters (bacteria, leucocytes and bacteria morphology). After applying the predictive algorithm, the UF‐1000i could optimally identify 95% urinary tract infections with high negative predictive value and low diagnostic error. Implementation of UF‐1000i would avoid culturing almost 38% of urine samples, thus reducing time to diagnosis, unnecessary antibiotic treatments and consequently improving cost‐effectiveness." @default.
• W2775604456 created "2017-12-22" @default.
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• W2775604456 date "2018-01-28" @default.
• W2775604456 modified "2023-09-27" @default.
• W2775604456 title "Sysmex UF-1000i flow cytometer to screen urinary tract infections: the URISCAM multicentre study" @default.
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• W2775604456 doi "https://doi.org/10.1111/lam.12832" @default.
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