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- W2775702772 abstract "The purpose of this study was to characterize the intrinsic cellular properties of orbital adipose-derived stem cells (OASC) from patients with thyroid-associated orbitopathy (TAO) and healthy controls.Orbital adipose tissue was collected from a total of nine patients: four controls and five patients with TAO. Isolated OASC were characterized with mesenchymal stem cell-specific markers. Orbital adipose-derived stem cells were differentiated into three lineages: chondrocytes, osteocytes, and adipocytes. Reverse transcription PCR of genes involved in the adipogenesis, chondrogenesis, and osteogenesis pathways were selected to assay the differentiation capacities. RNA sequencing analysis (RNA-seq) was performed and results were compared to assess for differences in gene expression between TAO and controls. Selected top-ranked results were confirmed by RT-PCR.Orbital adipose-derived stem cells isolated from orbital fat expressed high levels of mesenchymal stem cell markers, but low levels of the pluripotent stem cell markers. Orbital adipose-derived stem cells isolated from TAO patients exhibited an increase in adipogenesis, and a decrease in chondrogenesis and osteogenesis. RNA-seq disclosed 54 differentially expressed genes. In TAO OASC, expression of early neural crest progenitor marker (WNT signaling, ZIC genes and MSX2) was lost. Meanwhile, ectopic expression of HOXB2 and HOXB3 was found in the OASC from TAO.Our results suggest that there are intrinsic genetic and cellular differences in the OASC populations derived from TAO patients. The upregulation in adipogenesis in OASC of TAO may be is consistent with the clinical phenotype. Downregulation of early neural crest markers and ectopic expression of HOXB2 and HOXB3 in TAO OASC demonstrate dysregulation of developmental and tissue patterning pathways." @default.
- W2775702772 created "2017-12-22" @default.
- W2775702772 creator A5002609679 @default.
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- W2775702772 creator A5034794862 @default.
- W2775702772 creator A5082403692 @default.
- W2775702772 date "2017-12-06" @default.
- W2775702772 modified "2023-09-24" @default.
- W2775702772 title "RNA-Sequencing Gene Expression Profiling of Orbital Adipose-Derived Stem Cell Population Implicate <i>HOX</i> Genes and WNT Signaling Dysregulation in the Pathogenesis of Thyroid-Associated Orbitopathy" @default.
- W2775702772 cites W109421789 @default.
- W2775702772 cites W1485275533 @default.
- W2775702772 cites W1556057172 @default.
- W2775702772 cites W1608107506 @default.
- W2775702772 cites W1667206495 @default.
- W2775702772 cites W1900442171 @default.
- W2775702772 cites W1966487651 @default.
- W2775702772 cites W1969609924 @default.
- W2775702772 cites W1971482295 @default.
- W2775702772 cites W1977905463 @default.
- W2775702772 cites W1978627819 @default.
- W2775702772 cites W1985611882 @default.
- W2775702772 cites W1986352861 @default.
- W2775702772 cites W1986411614 @default.
- W2775702772 cites W1990125829 @default.
- W2775702772 cites W1996935786 @default.
- W2775702772 cites W2000818836 @default.
- W2775702772 cites W2003971855 @default.
- W2775702772 cites W2007453696 @default.
- W2775702772 cites W2011975727 @default.
- W2775702772 cites W2013545681 @default.
- W2775702772 cites W2016686483 @default.
- W2775702772 cites W2021249974 @default.
- W2775702772 cites W2022398371 @default.
- W2775702772 cites W2025851689 @default.
- W2775702772 cites W2031755982 @default.
- W2775702772 cites W2035478291 @default.
- W2775702772 cites W2041439225 @default.
- W2775702772 cites W2043129312 @default.
- W2775702772 cites W2051443513 @default.
- W2775702772 cites W2056815794 @default.
- W2775702772 cites W2058247907 @default.
- W2775702772 cites W2058466955 @default.
- W2775702772 cites W2059442774 @default.
- W2775702772 cites W2059574639 @default.
- W2775702772 cites W2061191160 @default.
- W2775702772 cites W2068040418 @default.
- W2775702772 cites W2069075566 @default.
- W2775702772 cites W2072447280 @default.
- W2775702772 cites W2077720819 @default.
- W2775702772 cites W2078120504 @default.
- W2775702772 cites W2081929389 @default.
- W2775702772 cites W2082596386 @default.
- W2775702772 cites W2090602346 @default.
- W2775702772 cites W2093134054 @default.
- W2775702772 cites W2101492172 @default.
- W2775702772 cites W2104844142 @default.
- W2775702772 cites W2106886193 @default.
- W2775702772 cites W2108130589 @default.
- W2775702772 cites W2108553386 @default.
- W2775702772 cites W2111034338 @default.
- W2775702772 cites W2118046075 @default.
- W2775702772 cites W2119635284 @default.
- W2775702772 cites W2122371847 @default.
- W2775702772 cites W2127053002 @default.
- W2775702772 cites W2141516235 @default.
- W2775702772 cites W2149483845 @default.
- W2775702772 cites W2150009399 @default.
- W2775702772 cites W2152493389 @default.
- W2775702772 cites W2153720415 @default.
- W2775702772 cites W2163219777 @default.
- W2775702772 cites W2174893517 @default.
- W2775702772 cites W2177415154 @default.
- W2775702772 cites W2184942504 @default.
- W2775702772 cites W2213706287 @default.
- W2775702772 cites W2320985696 @default.
- W2775702772 cites W2329041084 @default.
- W2775702772 cites W2414697164 @default.
- W2775702772 cites W2479713205 @default.
- W2775702772 cites W2575268794 @default.
- W2775702772 cites W2616562178 @default.
- W2775702772 cites W4248098588 @default.
- W2775702772 cites W4253074478 @default.
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- W2775702772 doi "https://doi.org/10.1167/iovs.17-22237" @default.
- W2775702772 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5718600" @default.
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- W2775702772 hasPublicationYear "2017" @default.
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