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- W2775821921 abstract "The aim of this study was to investigate the effect and mechanism by which progesterone regulates uterine contraction in late pregnant rats.Progesterone caused concentration-dependent relaxation of uterine strips that was enhanced compared with control nontreated uterine strips. Uterine strips incubated with progesterone showed a significant increase in TREK-1 mRNA expression and protein level. TREK-1 inhibitor L-methionine partly reversed uterine relaxation caused by the progesterone, while TREK-1 activator arachidonic acid did not cause significant change in progesterone-induced relaxation.Progesterone inhibits uterine contraction and induces uterine relaxation in late pregnancy. The progesterone-induced inhibition of uterine contraction appears to partly involve increased potassium channel TREK-1 expression/activity.Uterus from late-pregnant rats (gestational day 19) was isolated, and uterine strips were prepared for isometric contraction measurement. Oxytocin-induced contraction was compared in uterine strips pretreated with different concentration of progesterone. TREK-1 potassium channel inhibitor L-methionine and TREK-1 agonist arachidonic acid were used to determine whether the changes caused by progesterone involve changes in TREK-1 activity. The mRNA and protein expression of TREK-1 in uterine tissues were measured using qPCR and Western blot." @default.
- W2775821921 created "2017-12-22" @default.
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- W2775821921 date "2017-12-07" @default.
- W2775821921 modified "2023-10-12" @default.
- W2775821921 title "Progesterone inhibits contraction and increases TREK-1 potassium channel expression in late pregnant rat uterus" @default.
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- W2775821921 doi "https://doi.org/10.18632/oncotarget.23084" @default.
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