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- W2776160919 abstract "Two KP1(–) strains of Trypanosoma rangeli (SC-58, SC-61) isolated from the wild rodent Phyllomys dasythrix from SantaCatarina (Brazil) were compared with some KP1(+) reference stocks from different Latin America countries, and also with Trypanosomalewisi. The strains were analyzed by some morphological and biological features, and by biochemical and molecular techniques. The meantotal length (TL) of the bloodstream trypomastigotes of T. rangeli varied between 31.3–33.0 μm, and those of T. lewisi (adult forms) was28.2 μm, values within the variation known for each species. In T. rangeli KP1(+) and T. lewisi, the nucleus was located in the anterior portionof the body, with nuclear indexes (NI) ≥ 1.2, as typically described for both species. Differently, most trypomastigotes of the KP1(–)stocks presented NI ≤ 1.0. Another striking feature of the KP1(–) strains was their very fastidiously growth in axenic cultures when comparedwith the KP1(+) stocks and T. lewisi. Three isoenzyme loci (MDH, IDH and PGM) clearly distinguished T. rangeli and T. lewisi, andthe distinction between the KP1(+) and KP1(–) strains was possible at MDH, PGM and GPI loci. All T. rangeli strains presented the typical760 bp amplicon derived from their KP2 minicircles. However, the KP3 products of the KP1(+) strains were a single large band (~330bp),whereas those of the KP1(–) had two distinct bands (350 and 300 bp). T. lewisi presented 700 and 400 bp amplicons, as previously reported.The peculiarities of T. rangeli isolates from P. dasythrix corroborate a possible speciation process within this taxon." @default.
- W2776160919 created "2018-01-05" @default.
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- W2776160919 date "2017-01-01" @default.
- W2776160919 modified "2023-09-24" @default.
- W2776160919 title "Peculiarities of Trypanosoma rangeli KP1(-) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)" @default.
- W2776160919 doi "https://doi.org/10.4467/16890027ap.17.010.7485" @default.
- W2776160919 hasPublicationYear "2017" @default.
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