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- W2776258242 endingPage "e00571" @default.
- W2776258242 startingPage "e00571" @default.
- W2776258242 abstract "Type three secretion systems (T3SSs) are virulence determinants employed by several pathogenic bacteria as molecular syringes to inject effector proteins into host cells. Diarrhea-producing enteropathogenic Escherichia coli (EPEC) uses a T3SS to colonize the intestinal tract. T3S is a highly coordinated process that ensures hierarchical delivery of three classes of substrates: early (inner rod and needle subunits), middle (translocators), and late (effectors). Translocation of effectors is triggered upon host-cell contact in response to different environmental cues, such as calcium levels. The T3S substrate specificity switch from middle to late substrates in EPEC is regulated by the SepL and SepD proteins, which interact with each other and form a trimeric complex with the chaperone CesL. In this study, we investigated the link between calcium concentration and secretion regulation by the gatekeeper SepL. We found that calcium depletion promotes late substrate secretion in a translocon-independent manner. Furthermore, the stability, formation, and subcellular localization of the SepL/SepD/CesL regulatory complex were not affected by the absence of calcium. In addition, we demonstrate that SepL interacts in a calcium-independent manner with the major export gate component EscV, which in turn interacts with both middle and late secretion substrates, providing a docking site for T3S. These results suggest that EscV serves as a binding platform for both the SepL regulatory protein and secreted substrates during the ordered assembly of the T3SS." @default.
- W2776258242 created "2018-01-05" @default.
- W2776258242 creator A5020717545 @default.
- W2776258242 creator A5021239411 @default.
- W2776258242 creator A5028506726 @default.
- W2776258242 creator A5046915974 @default.
- W2776258242 creator A5068032364 @default.
- W2776258242 creator A5081786445 @default.
- W2776258242 creator A5091734906 @default.
- W2776258242 date "2017-12-26" @default.
- W2776258242 modified "2023-10-12" @default.
- W2776258242 title "Novel insights into the mechanism of SepL-mediated control of effector secretion in enteropathogenic<i>Escherichia coli</i>" @default.
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