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• W2776883406 abstract "This protocol was developed to increase the richness of information available from in vivo T cell proliferation studies. DNA labelling techniques such as BrdU incorporation allow precise control of label administration and withdrawal, so that the division history of a population can be tracked in detail over long timeframes (days-weeks). Ki67 is expressed in the nucleus of dividing cells, and is retained for a short time (3-4 days) after division ( Gossel et al., 2017 ); therefore acting as a molecular clock to identify cells that have recently divided. Combining these two techniques allows the integration of current and historical proliferation information from individual cells within a population. This data can subsequently be used to probe population dynamics by fitting mathematical models of proliferation ( Gossel et al., 2017 )." @default.
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• W2776883406 date "2017-01-01" @default.
• W2776883406 modified "2023-09-22" @default.
• W2776883406 title "Analysing Temporal Dynamics of T Cell Division in vivo Using Ki67 and BrdU Co-labelling by Flow Cytometry" @default.
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• W2776883406 doi "https://doi.org/10.21769/bioprotoc.2649" @default.
• W2776883406 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8438470" @default.
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