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- W2777165098 abstract "We synthesized a novel linker (1) with biotin, alkyne and amino groups for the identification of target proteins using a small molecule that contains an azide group (azide probe). The alkyne in the linker bound the azide probe via an azide-alkyne Huisgen cycloaddition. A protein cross-linker effectively bound the conjugate of the linker and an azide probe with a target protein. The covalently bound complex was detected by western blotting. Linker 1 was applied to a model system using an abscisic acid receptor, RCAR/PYR/PYL (PYL). Cross-linked complexes of linker 1, the azide probes and the target proteins were successfully visualized by western blotting. This method of target protein identification was more effective than a previously developed method that uses a second linker with biotin, alkyne, and benzophenone (linker 2) that acts to photo-crosslink target proteins. The system developed in this study is a method for identifying the target proteins of small bioactive molecules and is different from photo-affinity labelling." @default.
- W2777165098 created "2018-01-05" @default.
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- W2777165098 date "2018-02-01" @default.
- W2777165098 modified "2023-09-24" @default.
- W2777165098 title "Novel biotin linker with alkyne and amino groups for chemical labelling of a target protein of a bioactive small molecule" @default.
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- W2777165098 doi "https://doi.org/10.1016/j.bmcl.2017.12.055" @default.
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