Matches in SemOpenAlex for { <https://semopenalex.org/work/W2777283622> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2777283622 abstract "Sulfated polysaccharides constitute a large and complex group of macromolecules which possess a wide range of important biological properties. Many of them hold promise as new therapeutics, but determination of their blood levels during pharmacokinetic studies can be challenging. Heparin Red, a commercial mix-and-read fluorescence assay, has recently emerged as a tool in clinical drug development and pharmacokinetic analysis for the quantification of sulfated polysaccharides in human plasma. The present study describes the application of Heparin Red to the detection of heparin, a highly sulfated polysaccharide, and fucoidan, a less sulfated polysaccharide, in spiked mouse and rat plasmas. While the standard assay protocol for human plasma matrix gave less satisfactory results, a modified protocol was developed that provides within a detection range 0 to 10 micrograms per mL better limits of quantification, 1.1 to 2.3 micrograms per mL for heparin, and 1.7 to 3.4 micrograms per mL for fucoidan. The required plasma sample volume of only 20 microliters is advantegous in particular when blood samples need to be collected from mice. Our results suggest that Heparin Red is a promising tool for the preclinical evaluation of sulfated polysaccharides with varying sulfation degrees in mouse and rat models." @default.
- W2777283622 created "2018-01-05" @default.
- W2777283622 creator A5015951745 @default.
- W2777283622 creator A5067571033 @default.
- W2777283622 creator A5071318798 @default.
- W2777283622 date "2017-12-15" @default.
- W2777283622 modified "2023-09-27" @default.
- W2777283622 title "Quantification of sulfated polysaccharides in mouse and rat plasma by the Heparin Red mix-and-read fluorescence assay" @default.
- W2777283622 cites W1633091337 @default.
- W2777283622 cites W1969297934 @default.
- W2777283622 cites W1973803139 @default.
- W2777283622 cites W2038871057 @default.
- W2777283622 cites W2052951555 @default.
- W2777283622 cites W2068212194 @default.
- W2777283622 cites W2121179180 @default.
- W2777283622 cites W2124145724 @default.
- W2777283622 cites W2161098783 @default.
- W2777283622 cites W2418877261 @default.
- W2777283622 cites W2471047817 @default.
- W2777283622 cites W2495245775 @default.
- W2777283622 cites W2521461699 @default.
- W2777283622 cites W2585647019 @default.
- W2777283622 cites W2606401822 @default.
- W2777283622 cites W2610898931 @default.
- W2777283622 cites W2612899996 @default.
- W2777283622 cites W2702177848 @default.
- W2777283622 cites W2760987893 @default.
- W2777283622 cites W2772229718 @default.
- W2777283622 cites W2780517818 @default.
- W2777283622 cites W2917703340 @default.
- W2777283622 hasPublicationYear "2017" @default.
- W2777283622 type Work @default.
- W2777283622 sameAs 2777283622 @default.
- W2777283622 citedByCount "1" @default.
- W2777283622 countsByYear W27772836222018 @default.
- W2777283622 crossrefType "posted-content" @default.
- W2777283622 hasAuthorship W2777283622A5015951745 @default.
- W2777283622 hasAuthorship W2777283622A5067571033 @default.
- W2777283622 hasAuthorship W2777283622A5071318798 @default.
- W2777283622 hasConcept C100817775 @default.
- W2777283622 hasConcept C112705442 @default.
- W2777283622 hasConcept C155138218 @default.
- W2777283622 hasConcept C185592680 @default.
- W2777283622 hasConcept C2775909754 @default.
- W2777283622 hasConcept C2777557582 @default.
- W2777283622 hasConcept C3020134792 @default.
- W2777283622 hasConcept C43617362 @default.
- W2777283622 hasConcept C55493867 @default.
- W2777283622 hasConcept C86803240 @default.
- W2777283622 hasConcept C98274493 @default.
- W2777283622 hasConceptScore W2777283622C100817775 @default.
- W2777283622 hasConceptScore W2777283622C112705442 @default.
- W2777283622 hasConceptScore W2777283622C155138218 @default.
- W2777283622 hasConceptScore W2777283622C185592680 @default.
- W2777283622 hasConceptScore W2777283622C2775909754 @default.
- W2777283622 hasConceptScore W2777283622C2777557582 @default.
- W2777283622 hasConceptScore W2777283622C3020134792 @default.
- W2777283622 hasConceptScore W2777283622C43617362 @default.
- W2777283622 hasConceptScore W2777283622C55493867 @default.
- W2777283622 hasConceptScore W2777283622C86803240 @default.
- W2777283622 hasConceptScore W2777283622C98274493 @default.
- W2777283622 hasLocation W27772836221 @default.
- W2777283622 hasOpenAccess W2777283622 @default.
- W2777283622 hasPrimaryLocation W27772836221 @default.
- W2777283622 hasRelatedWork W1041970429 @default.
- W2777283622 hasRelatedWork W1965265193 @default.
- W2777283622 hasRelatedWork W2011959743 @default.
- W2777283622 hasRelatedWork W2045760362 @default.
- W2777283622 hasRelatedWork W2076806500 @default.
- W2777283622 hasRelatedWork W2090519890 @default.
- W2777283622 hasRelatedWork W2092017537 @default.
- W2777283622 hasRelatedWork W2103061389 @default.
- W2777283622 hasRelatedWork W2159833218 @default.
- W2777283622 hasRelatedWork W2331437292 @default.
- W2777283622 hasRelatedWork W2349883460 @default.
- W2777283622 hasRelatedWork W2377775581 @default.
- W2777283622 hasRelatedWork W2556257311 @default.
- W2777283622 hasRelatedWork W2592337340 @default.
- W2777283622 hasRelatedWork W2942571621 @default.
- W2777283622 hasRelatedWork W299208096 @default.
- W2777283622 hasRelatedWork W3000672360 @default.
- W2777283622 hasRelatedWork W905027267 @default.
- W2777283622 hasRelatedWork W106110696 @default.
- W2777283622 hasRelatedWork W2757156476 @default.
- W2777283622 isParatext "false" @default.
- W2777283622 isRetracted "false" @default.
- W2777283622 magId "2777283622" @default.
- W2777283622 workType "article" @default.