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• W2777391621 abstract "Introduction: Continuous use of opiates causes drug-related illnesses, which poses an alarming situation to develop sensitive detection platform. In this study, a highly sensitive and reliable chemiluminescence immunoassay (CI) has been developed for the detection of heroin and its major metabolites in spiked urine samples. Methods: To develop robust immunoassay, monoacetyl morphine-bovine serum albumin (MAM-BSA) conjugate was synthesized and characterized thoroughly by physicochemical techniques. The anti-MAM antibodies were developed, labeled with horseradish peroxidase (HRP) and immunoassay was developed to detect the presence of target drug in spiked urine samples. Results: A competitive CI was developed, where heroin, MAM, morphine, and codeine concentration were ranged from 0-1000 ng/ mL in spiked urine samples and limit of detection were 80, 95, 90, 75 pg/ mL. Conclusion: The developed CI is highly sensitive, specific, point of care, cost-effective and can be used as a routine technique for quantitative analysis for screening of narcotic drugs." @default.
• W2777391621 created "2018-01-05" @default.
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• W2777391621 date "2017-10-30" @default.
• W2777391621 modified "2023-10-15" @default.
• W2777391621 title "Chemiluminescence based immunoassay for the detection of heroin and its metabolites" @default.
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• W2777391621 doi "https://doi.org/10.15171/bi.2018.07" @default.
• W2777391621 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5915709" @default.
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