FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2777691899> ?p ?o ?g. }

• W2777691899 endingPage "73" @default.
• W2777691899 startingPage "59" @default.
• W2777691899 abstract "The female reproductive tract (FRT) presents a unique challenge to the mucosal immune system as it needs to monitor constantly for the presence of opportunistic pathogens amidst its commensal flora. During infection, autophagy plays a critical role in pathogen clearance, presentation of antigens and production of pro-inflammatory cytokines. However, no information is available that describes the role of autophagy in mouse vaginal infection of Candida albicans. The objective of our study is to evaluate the effect of autophagy gene, ATG5 knockout in vaginal cells in response to vaginal C. albicans infection. Mice having knockout of ATG5 in the vaginal cells (PR-ATG5-KO mice) were infected intra-vaginally with the yeast form of Candida albicans. Vaginal lavages were collected once in a week until the infection was cleared. We detected the expression of autophagy marker genes (LC3, ATG5 and LAMP1) in the vaginal cells. We determined the levels of various cytokines (IL-1α, IL-1β, IL-6, IL-10, IL-17A, IL-22, IL-23p19, TNF-α and G-CSF) involved in anti-candida response. The levels of cytokines in the vaginal lavages were quantified using Aimplex Premixed analyte kit. The vaginal lavages were checked for polymorphonuclear leucocytes (PMNLs) infiltration. The candida clearance rate from the vaginal lumen was determined by Colony Forming Units (CFUs) assay. The results revealed that PR-ATG5-KO mice failed to induce the expression of LC3, ATG5 and LAMP1 indicating an impaired autophagy pathway. The levels of all the cytokines (except IL-10) in C. albicans infected PR-ATG5-KO mice were significantly reduced as compared to the wild type infected C57BL/6 mice. The number of PMNLs infiltrated into the vaginal lavages of infected PR-ATG5-KO mice was reduced. The clearance of C. albicans from the vaginal lumen was also considerably delayed in PR-ATG5-KO mice. In conclusion, the results revealed that impaired autophagy in vaginal cells influences host response during vaginal infection of C. albicans by affecting anti-Candida cytokine levels in the vaginal lavage resulting in reduction of pathogen clearance rate." @default.
• W2777691899 created "2018-01-05" @default.
• W2777691899 creator A5000710376 @default.
• W2777691899 creator A5010573225 @default.
• W2777691899 creator A5081874939 @default.
• W2777691899 creator A5081973084 @default.
• W2777691899 date "2018-02-01" @default.
• W2777691899 modified "2023-09-25" @default.
• W2777691899 title "Knockout of autophagy gene, ATG5 in mice vaginal cells abrogates cytokine response and pathogen clearance during vaginal infection of Candida albicans" @default.
• W2777691899 cites W1559752786 @default.
• W2777691899 cites W1627009127 @default.
• W2777691899 cites W1964565569 @default.
• W2777691899 cites W1980201642 @default.
• W2777691899 cites W1987625474 @default.
• W2777691899 cites W1992881494 @default.
• W2777691899 cites W2006714910 @default.
• W2777691899 cites W2012443484 @default.
• W2777691899 cites W2023382400 @default.
• W2777691899 cites W2023856845 @default.
• W2777691899 cites W2029491405 @default.
• W2777691899 cites W2032700869 @default.
• W2777691899 cites W2045134338 @default.
• W2777691899 cites W2060067993 @default.
• W2777691899 cites W2067692987 @default.
• W2777691899 cites W2068713513 @default.
• W2777691899 cites W2070171027 @default.
• W2777691899 cites W2070418581 @default.
• W2777691899 cites W2073729562 @default.
• W2777691899 cites W2079550192 @default.
• W2777691899 cites W2081057817 @default.
• W2777691899 cites W2088140877 @default.
• W2777691899 cites W2100918356 @default.
• W2777691899 cites W2107235439 @default.
• W2777691899 cites W2107277218 @default.
• W2777691899 cites W2112133235 @default.
• W2777691899 cites W2123093300 @default.
• W2777691899 cites W2124826696 @default.
• W2777691899 cites W2128537698 @default.
• W2777691899 cites W2134900294 @default.
• W2777691899 cites W2159754384 @default.
• W2777691899 cites W2160312983 @default.
• W2777691899 cites W2167495346 @default.
• W2777691899 cites W2170016098 @default.
• W2777691899 cites W2322223176 @default.
• W2777691899 cites W2336308267 @default.
• W2777691899 cites W2587409612 @default.
• W2777691899 doi "https://doi.org/10.1016/j.cellimm.2017.12.012" @default.
• W2777691899 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29306553" @default.
• W2777691899 hasPublicationYear "2018" @default.
• W2777691899 type Work @default.
• W2777691899 sameAs 2777691899 @default.
• W2777691899 citedByCount "11" @default.
• W2777691899 countsByYear W27776918992018 @default.
• W2777691899 countsByYear W27776918992019 @default.
• W2777691899 countsByYear W27776918992020 @default.
• W2777691899 countsByYear W27776918992022 @default.
• W2777691899 countsByYear W27776918992023 @default.
• W2777691899 crossrefType "journal-article" @default.
• W2777691899 hasAuthorship W2777691899A5000710376 @default.
• W2777691899 hasAuthorship W2777691899A5010573225 @default.
• W2777691899 hasAuthorship W2777691899A5081874939 @default.
• W2777691899 hasAuthorship W2777691899A5081973084 @default.
• W2777691899 hasConcept C190283241 @default.
• W2777691899 hasConcept C203014093 @default.
• W2777691899 hasConcept C203522944 @default.
• W2777691899 hasConcept C2776460866 @default.
• W2777691899 hasConcept C2780917455 @default.
• W2777691899 hasConcept C2781000418 @default.
• W2777691899 hasConcept C55493867 @default.
• W2777691899 hasConcept C86803240 @default.
• W2777691899 hasConcept C8891405 @default.
• W2777691899 hasConcept C89423630 @default.
• W2777691899 hasConceptScore W2777691899C190283241 @default.
• W2777691899 hasConceptScore W2777691899C203014093 @default.
• W2777691899 hasConceptScore W2777691899C203522944 @default.
• W2777691899 hasConceptScore W2777691899C2776460866 @default.
• W2777691899 hasConceptScore W2777691899C2780917455 @default.
• W2777691899 hasConceptScore W2777691899C2781000418 @default.
• W2777691899 hasConceptScore W2777691899C55493867 @default.
• W2777691899 hasConceptScore W2777691899C86803240 @default.
• W2777691899 hasConceptScore W2777691899C8891405 @default.
• W2777691899 hasConceptScore W2777691899C89423630 @default.
• W2777691899 hasLocation W27776918991 @default.
• W2777691899 hasLocation W27776918992 @default.
• W2777691899 hasOpenAccess W2777691899 @default.
• W2777691899 hasPrimaryLocation W27776918991 @default.
• W2777691899 hasRelatedWork W1238671599 @default.
• W2777691899 hasRelatedWork W2130682212 @default.
• W2777691899 hasRelatedWork W2790584093 @default.
• W2777691899 hasRelatedWork W3046504410 @default.
• W2777691899 hasRelatedWork W3107998850 @default.
• W2777691899 hasRelatedWork W4205192361 @default.
• W2777691899 hasRelatedWork W4285012569 @default.
• W2777691899 hasRelatedWork W4367318058 @default.
• W2777691899 hasRelatedWork W977480810 @default.
• W2777691899 hasRelatedWork W2313463430 @default.
• W2777691899 hasVolume "324" @default.
• W2777691899 isParatext "false" @default.

• next