FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2777882990> ?p ?o ?g. }

• W2777882990 endingPage "e0190240" @default.
• W2777882990 startingPage "e0190240" @default.
• W2777882990 abstract "Campylobacter jejuni and C. coli are important food-borne pathogens that are widespread in animal husbandry. To combat Campylobacter along the food chain, the application of lytic phages has been shown to be a promising tool. Campylobacter phages are currently classified into three groups, of which group II and group III phages are the most common. Members of each group are closely related, whereas the two groups share only little DNA similarity. Moreover, while group III phages are specific for C. jejuni, group II phages additionally infect C. coli. Phage cocktails intended to be used for applications should be composed of various phages that differ in their host range and growth kinetics. The isolation of phages is generally performed by plaque assays. This approach has the limitation that phages are merely identified by their lytic activity on certain indicator strains and that relatively high numbers of phages must be present in a tested sample. Therefore, a more sensitive molecular detection system would be beneficial, which allows a pre-screening of samples and the quick detection and discrimination of group II and group III phages. New phages can then be isolated by use of indicator strains that may be different from those typically applied. On the basis of available Campylobacter phage genome sequences, we developed a multiplex PCR system for group II and group III phages selecting the tail tube gene and the gene for the base plate wedge, respectively, as target. Phages of both groups could be identified with primers deduced from the putative tail fiber gene. Efficient release of phage DNA from capsids was achieved by an extended heat treatment or digestion of phage particles with proteinase K/SDS yielding a detection limit of 1 pfu/ml. Individual detection of group II phages, group III phages and of both groups was studied with artificially contaminated chicken skin. To recover phages that had strongly adhered to the skin, stomaching was the most efficient technique. The developed PCR protocol was employed to detect Campylobacter phages in food and environmental samples. In 50 out of 110 samples group II and/or group III phages were identified." @default.
• W2777882990 created "2018-01-05" @default.
• W2777882990 creator A5045645321 @default.
• W2777882990 creator A5053222538 @default.
• W2777882990 creator A5081488412 @default.
• W2777882990 creator A5090524004 @default.
• W2777882990 date "2017-12-22" @default.
• W2777882990 modified "2023-09-25" @default.
• W2777882990 title "A multiplex real-time PCR for the detection and differentiation of Campylobacter phages" @default.
• W2777882990 cites W1496270097 @default.
• W2777882990 cites W1550682512 @default.
• W2777882990 cites W1820579908 @default.
• W2777882990 cites W1964651715 @default.
• W2777882990 cites W196513295 @default.
• W2777882990 cites W1965177448 @default.
• W2777882990 cites W1975884269 @default.
• W2777882990 cites W2012482159 @default.
• W2777882990 cites W2024142244 @default.
• W2777882990 cites W2030448230 @default.
• W2777882990 cites W2046180712 @default.
• W2777882990 cites W2066289193 @default.
• W2777882990 cites W2078369332 @default.
• W2777882990 cites W2081568552 @default.
• W2777882990 cites W2093661338 @default.
• W2777882990 cites W2095557713 @default.
• W2777882990 cites W2097044860 @default.
• W2777882990 cites W2107792352 @default.
• W2777882990 cites W2111047368 @default.
• W2777882990 cites W2111619448 @default.
• W2777882990 cites W2113625817 @default.
• W2777882990 cites W2113855069 @default.
• W2777882990 cites W2139779127 @default.
• W2777882990 cites W2144225596 @default.
• W2777882990 cites W2144443179 @default.
• W2777882990 cites W2152005348 @default.
• W2777882990 cites W2162098634 @default.
• W2777882990 cites W2168366858 @default.
• W2777882990 cites W2171403089 @default.
• W2777882990 cites W2184320396 @default.
• W2777882990 cites W2462447455 @default.
• W2777882990 cites W2555946358 @default.
• W2777882990 cites W4244464723 @default.
• W2777882990 cites W75335755 @default.
• W2777882990 doi "https://doi.org/10.1371/journal.pone.0190240" @default.
• W2777882990 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5741259" @default.
• W2777882990 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29272305" @default.
• W2777882990 hasPublicationYear "2017" @default.
• W2777882990 type Work @default.
• W2777882990 sameAs 2777882990 @default.
• W2777882990 citedByCount "11" @default.
• W2777882990 countsByYear W27778829902018 @default.
• W2777882990 countsByYear W27778829902019 @default.
• W2777882990 countsByYear W27778829902020 @default.
• W2777882990 countsByYear W27778829902021 @default.
• W2777882990 countsByYear W27778829902022 @default.
• W2777882990 crossrefType "journal-article" @default.
• W2777882990 hasAuthorship W2777882990A5045645321 @default.
• W2777882990 hasAuthorship W2777882990A5053222538 @default.
• W2777882990 hasAuthorship W2777882990A5081488412 @default.
• W2777882990 hasAuthorship W2777882990A5090524004 @default.
• W2777882990 hasBestOaLocation W27778829901 @default.
• W2777882990 hasConcept C104317684 @default.
• W2777882990 hasConcept C141231307 @default.
• W2777882990 hasConcept C159047783 @default.
• W2777882990 hasConcept C182615771 @default.
• W2777882990 hasConcept C2522874641 @default.
• W2777882990 hasConcept C2776441376 @default.
• W2777882990 hasConcept C2776957140 @default.
• W2777882990 hasConcept C2779910956 @default.
• W2777882990 hasConcept C2781188995 @default.
• W2777882990 hasConcept C49105822 @default.
• W2777882990 hasConcept C523546767 @default.
• W2777882990 hasConcept C54355233 @default.
• W2777882990 hasConcept C547475151 @default.
• W2777882990 hasConcept C73445445 @default.
• W2777882990 hasConcept C86803240 @default.
• W2777882990 hasConcept C89423630 @default.
• W2777882990 hasConcept C90583042 @default.
• W2777882990 hasConceptScore W2777882990C104317684 @default.
• W2777882990 hasConceptScore W2777882990C141231307 @default.
• W2777882990 hasConceptScore W2777882990C159047783 @default.
• W2777882990 hasConceptScore W2777882990C182615771 @default.
• W2777882990 hasConceptScore W2777882990C2522874641 @default.
• W2777882990 hasConceptScore W2777882990C2776441376 @default.
• W2777882990 hasConceptScore W2777882990C2776957140 @default.
• W2777882990 hasConceptScore W2777882990C2779910956 @default.
• W2777882990 hasConceptScore W2777882990C2781188995 @default.
• W2777882990 hasConceptScore W2777882990C49105822 @default.
• W2777882990 hasConceptScore W2777882990C523546767 @default.
• W2777882990 hasConceptScore W2777882990C54355233 @default.
• W2777882990 hasConceptScore W2777882990C547475151 @default.
• W2777882990 hasConceptScore W2777882990C73445445 @default.
• W2777882990 hasConceptScore W2777882990C86803240 @default.
• W2777882990 hasConceptScore W2777882990C89423630 @default.
• W2777882990 hasConceptScore W2777882990C90583042 @default.
• W2777882990 hasIssue "12" @default.
• W2777882990 hasLocation W27778829901 @default.
• W2777882990 hasLocation W27778829902 @default.

• next