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• W2778531845 abstract "Benefiting from the specially tailored properties of the building blocks as well as of the scaffolds, DNA-assembled core–satellite superstructures have gained increasing interest in drug delivery, imaging, and biosensing. The load of satellites plays a vital role in core–satellite superstructures, and it determines the signal intensity in response to a biological/physical stimulation/actuation. Herein, for the first time, we utilize on-particle rolling circle amplification (RCA) to prepare rapidly responsive core–satellite magnetic superstructures with a high load of magnetic nanoparticle (MNP) satellites. Combined with duplex-specific nuclease-assisted target recycling, the proposed magnetic superstructures hold great promise in sensitive and rapid microRNA detection. The long single-stranded DNA produced by RCA serving as the scaffold of the core–satellite superstructure can be hydrolyzed by duplex-specific nuclease in the presence of target microRNA, resulting in a release of MNPs that can be quantified in an optomagnetic sensor. The proposed biosensor has a simple mix–separate–measure strategy. For let-7b detection, the proposed biosensor offers a wide linear detection range of approximately 5 orders of magnitude with a detection sensitivity of 1 fM. Moreover, it has the capability to discriminate single-nucleotide mismatches and to detect let-7b in cell extracts and serum, thus showing considerable potential for clinical applications." @default.
• W2778531845 created "2018-01-05" @default.
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• W2778531845 date "2018-01-08" @default.
• W2778531845 modified "2023-10-11" @default.
• W2778531845 title "On-Particle Rolling Circle Amplification-Based Core–Satellite Magnetic Superstructures for MicroRNA Detection" @default.
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• W2778531845 doi "https://doi.org/10.1021/acsami.7b16293" @default.
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